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Series GSE32120 Query DataSets for GSE32120
Status Public on Dec 22, 2011
Title The little elongation complex (LEC) regulates small nuclear RNA transcription
Organisms Drosophila melanogaster; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by array
Summary The Eleven-nineteen Lysine-rich Leukemia (ELL)-containing Super Elongation Complex (SEC) containing P-TEFb is a key regulator in the expression of HOX genes in Mixed Lineage Leukemia (MLL)-based leukemia. We have identified an SEC-like complex in Drosophila, as well as a distinct ELL-containing complex that lacks P-TEFb and other components of SEC named the "little elongation complex" (LEC). LEC subunits are highly enriched at RNA Polymerase II (Pol II) transcribed small nuclear RNA (snRNA) genes and the loss of LEC results in decreased snRNA expression in both flies and mammals. The discovery of specificity of SEC and LEC complexes for mRNA and snRNA containing genes, respectively, suggest the presence of specific classes of elongation factors for each class of genes transcribed by RNA polymerase II.
Overall design Examination of genome-wide binding profiles for ELL, Ice1, Lilli, and Pol II in D. melanogaster and by ChIP-seq. Identification of differentially expressed genes in ELL-RNAi and Ice1-RNAi in D. melanogaster by RNA-seq. Examination of genome-wide binding profiles for ELL in M. musculus. Identification of differentially expressed genes in ELL-RNAi in M. musculus by RNA-seq.
Contributor(s) Garrett AS, Smith ES, Shilatifard A
Citation(s) 22195968
Submission date Sep 14, 2011
Last update date May 15, 2019
Contact name Alexander (Garrett) Garruss
Organization name Stowers Institute for Medical Research
Lab Shilatifard
Street address 1000 East 50th Street
City Kansas CIty
State/province Missouri
ZIP/Postal code 64110
Country USA
Platforms (4)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL11203 Illumina Genome Analyzer IIx (Drosophila melanogaster)
Samples (22)
GSM796328 ELL ChIP-Seq
GSM796329 Ice1 ChIP-Seq
GSM796330 Lilli ChIP-Seq
BioProject PRJNA147449
SRA SRP008264

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32120_RAW.tar 5.0 Gb (http)(custom) TAR (of BED, CEL)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data included within Sample table
Processed data provided as supplementary file

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