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| Status |
Public on Dec 01, 2011 |
| Title |
Gene expression signatures in GIST-T1 cells transfected with siRNA targeting HOTAIR |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To analyze the effect of HOTAIR depletion in a gastrointestinal stromal tumor (GIST) cells, RNAi-mediated knockdown of HOTAIR was carried out using GIST-T1 cells. Gene expression microarray analysis revealed that a number of reported HOTAIR target genes were upregulated by knockdown of HOTAIR. Moreover, we found that 1424 genes were upregulated by siHOT (>2-fold), and Gene Ontology analysis revealed enrichment of genes related to “nucleus”, “chromosome” and “membrane-bounded organelle.”
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| Overall design |
For RNAi-mediated knockdown of HOTAIR, three different Stealth siRNAs against HOTAIR were generated by Invitrogen, after which a mixture of the three was used for transfection. GIST-T1 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen). Total RNA was extracted 48 h after transfection.
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| Contributor(s) |
Suzuki H, Shinomura Y |
| Citation(s) |
22258453 |
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| Submission date |
Sep 12, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
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| Phone |
+81-11-611-2111
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| Organization name |
Sapporo Medical University
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| Department |
Department of Molecular Biology
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| Street address |
S1, W17, Chuo-ku
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| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA147601 |
