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| Status |
Public on Jan 03, 2013 |
| Title |
Widespread regulation of translation by elongation pausing in heat shock |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
RNA-seq and ribosome footprinting libraries of mouse 3T3 and human 293T cellsrelated to Shalgi et al. 2013
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| Overall design |
36bases paired-end RNA-seq, and ribosome footprinting libraries for: 3T3 cells - Control, 8 hours of mild heat shock (42) and 2 hours of severe heat shock (44) - in replicates, as well as 3T3 cells treated by mild followed by severe heat shock. In addition, 3T3 cells treated with Hsp70 inhibitor VER-155008 (Massey et al. 2010), and 293T cells transfected with Hspa1a or GFP, before and after 2 hours of severe heat shock.
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| |
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| Contributor(s) |
Shalgi R, Hurt JA, Krykbaeva I, Taipale M, Lindquist S, Burge CB |
| Citation(s) |
23290915 |
| Submission date |
Sep 12, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Reut Shalgi |
| E-mail(s) |
reutshalgi@technion.ac.il
|
| Organization name |
Technion
|
| Department |
Faculty of Medicine
|
| Street address |
1 Efron st.
|
| City |
Haifa |
| ZIP/Postal code |
31096 |
| Country |
Israel |
| |
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| Platforms (3) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA147613 |
| SRA |
SRP017942 |
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