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Series GSE32060 Query DataSets for GSE32060
Status Public on Jan 03, 2013
Title Widespread regulation of translation by elongation pausing in heat shock
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA-seq and ribosome footprinting libraries of mouse 3T3 and human 293T cellsrelated to Shalgi et al. 2013
Overall design 36bases paired-end RNA-seq, and ribosome footprinting libraries for: 3T3 cells - Control, 8 hours of mild heat shock (42) and 2 hours of severe heat shock (44) - in replicates, as well as 3T3 cells treated by mild followed by severe heat shock. In addition, 3T3 cells treated with Hsp70 inhibitor VER-155008 (Massey et al. 2010), and 293T cells transfected with Hspa1a or GFP, before and after 2 hours of severe heat shock.
Contributor(s) Shalgi R, Hurt JA, Krykbaeva I, Taipale M, Lindquist S, Burge CB
Citation(s) 23290915
Submission date Sep 12, 2011
Last update date May 15, 2019
Contact name Reut Shalgi
Organization name Technion
Department Faculty of Medicine
Street address 1 Efron st.
City Haifa
ZIP/Postal code 31096
Country Israel
Platforms (3)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM794848 3T3-Control-RNAseq
GSM794849 3T3-8hrs-mild-HS-RNAseq
GSM794850 3T3-2hrs-severe-HS-RNAseq
BioProject PRJNA147613
SRA SRP017942

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32060_Control_RNA.combined.bowtie.sam.gz 1.1 Gb (ftp)(http) SAM
GSE32060_HS2S_RNA.combined.bowtie.sam.gz 980.7 Mb (ftp)(http) SAM
GSE32060_HS8M_RNA.combined.bowtie.sam.gz 1.4 Gb (ftp)(http) SAM
GSE32060_RAW.tar 6.7 Gb (http)(custom) TAR (of BED, SAM)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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