NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE3202 Query DataSets for GSE3202
Status Public on Sep 10, 2005
Title MK886 treatment of H720 non-small cell lung cancer cell line
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this experiments different treatments were applied to lung cancer cell lines

1)H720 cell line suspension was treated by 1 micromole of 5-lipoxygenase activating protein inhibitor MK886 serum-free in TIS medium for 24 h.
2) A549 cell line was treated as H720 by MK886
3) A549 was treated by 100 nM of silencing cocktail (siRNA) prepared against the 4-th exon of the gene and generated by RNASEIII digestion of double stranded RNA. The cocktail was prepared by Ambion technology, see the manufacturer's protocol. The observed silencing was >90%
4) Based on Oligoengine vectors, silencing constructs were created against HRPA2B1 transcript(HNRPA2B1 is a subunit of spliceosome). Stable transfectants were raised harboring the construct.
Keywords: drug treatment
 
Overall design 1) MK886 treatement of H720: Three chips titled MK served as "treated" part, three chips titled "control" served as controls.
2) MK886 treatment of A549: two duplicates, 1+2 and 3+4, performed on different dates
3) HNRPA2B1 silencing: pairs of controls and silenced sample
4) FLAP silencing: 1 control, 2 treatments
 
Contributor(s) Mayburd AL
Citation(s) 16551867
Submission date Aug 25, 2005
Last update date Mar 25, 2019
Contact name Anatoly L. Mayburd
E-mail(s) mayburna@mail.nih.gov
Phone 1-301-402-3308
Organization name NIH/NCI
Department Intervention section
Lab Mulshine
Street address 9000 Rockville Pyke
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (23)
GSM71936 H720 cell line control 2
GSM71937 H720 cell line control 3
GSM71938 H720 cell line control 4
Relations
BioProject PRJNA92869

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3202_RAW.tar 1.6 Gb (http)(custom) TAR (of CEL, CHP, DAT, RPT)
Processed data provided as supplementary file
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap