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| Status |
Public on Sep 13, 2011 |
| Title |
Verification of a custom chip which measures abundance, isoforms and alleles in Drosophila |
| Organism |
Drosophila simulans |
| Experiment type |
Expression profiling by array
Other
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| Summary |
Analysis of differential expression using the 3’ expression module revealed 34% probe sets showed evidence for a sex bias and alternative exon sex bias was identified for 164 genes. Intriguingly, allelic imbalance was detected for 37% of 6,579 probe sets examined which contained heterozygous SNP loci.
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| Overall design |
Two different isogenic strains of D. simulans, st e and C167.4 and their male and female progeny were used as the basis for the verification study. Three replicates of RNA from female and male progeny of the cross st e x C167.4 made for a total of six RNA samples. In addition, DNA was used as a control for estimating allele imbalance. Three replicates of DNA target were prepared for female st e, femaleC167.4 and the female F1 progeny of the cross st e x C167.4, for a total of 9 gDNA samples.
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| Contributor(s) |
Yang Y, Graze RM, Waltz BM, Lopez C, Baker HV, Wayne ML, Nuzhdin S, McIntyre LM |
| Citation(s) |
24696400 |
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Yajie Yang, Rita M. Graze, Brandon M. Walts, Cecilia M. Lopez, Henry V. Baker, Marta L. Wayne, Sergey V. Nuzhdin, and Lauren M. McIntyre. Partitioning Transcript Variation in Drosophila: Abundance, Isoforms, and Alleles. G3 November 2011 1:427-436; doi:10.1534/g3.111.000596
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| Submission date |
Aug 30, 2011 |
| Last update date |
Sep 17, 2014 |
| Contact name |
Yajie Yang |
| E-mail(s) |
yyj920@gmail.com
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| Organization name |
UF
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| Street address |
2033 Mowry Road Room 118
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA145327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE31750_RAW.tar |
533.9 Mb |
(http)(custom) |
TAR (of CEL, CSV) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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