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Series GSE31620 Query DataSets for GSE31620
Status Public on Dec 01, 2011
Title MicroRNA-1 is a candidate tumor suppressor and prognostic marker
Platform organisms Homo sapiens; Mus musculus
Sample organism Homo sapiens
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary MicroRNAs (miRs) are small non-coding RNAs that can function as tumor suppressor genes. We previously reported that miR-1 is among the most consistently down-regulated miRs in primary human prostate tumors. In this follow-up study, we further corroborated this finding in an independent dataset and made the novel observation that miR-1 expression is further reduced in distant metastasis and is a predictor of disease recurrence. Moreover, we performed in vitro experiments to explore the candidate tumor suppressor function of miR-1. Cell-based assays showed that miR-1 is epigenetically silenced in human prostate cancer cells. Overexpression of miR-1 in these cells led to growth inhibition and down-regulation of genes in pathways regulating cell cycle progression, mitosis, DNA replication/repair, and actin dynamics. This observation was further corroborated with protein expression analysis and 3’-UTR-based reporter assays, indicating that genes in these pathways are either direct or indirect targets of miR-1. A gene set enrichment analysis revealed that miR-1-mediated tumor suppressor effects are globally similar to those of histone deacetylase inhibitors. Lastly, we obtained preliminary evidence that miR-1 alters gH2A.X marker expression and affects the cellular organization of F-actin and filipodia formation. In conclusion, our findings indicate that miR-1 acts as a tumor suppressor in prostate cancer by influencing multiple cancer-related processes and by inhibiting cell proliferation and motility.
Overall design In this study we monitored global miRNA expression changes in prostate cancer LNCaP cells treated with the epigenetic compounds 5-Azacytidine (5-AzaC) and/or trichostatin A (TSA). Cells were treated with epigenetic drugs for 36 hours and total RNA was isolated for hybridization to miRNA microarrays. 5 independent experiments were performed (n=4 for combined treatment).

The candidate prostate tumor suppressor miRNAs, miR-1, miR-206, and miR-27 were up-regulated in LNCaP cells for Affymetrix microarray analysis. LNCaP cells were transfected with pre-miR oligos and 24 hr post-transfection total RNA was collected for microarray analysis; total of three independent experiments.
Contributor(s) Hudson RS, Ambs S, Yi M
Citation(s) 22210864, 23409773
Submission date Aug 23, 2011
Last update date Sep 17, 2019
Contact name Robert Scott Hudson
Organization name NIH
Department NCI
Lab The Laboratory of Human Carcinogenesis
Street address National Cancer Institute Room Blgd.37/3044
City Bethesda
State/province MD
ZIP/Postal code 20852
Country USA
Platforms (2)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
GPL14184 OSU-CCC Human and Mouse MicroRNA Microarray Version 4.0
Samples (34)
GSM785477 pre-miR-control 1
GSM785478 pre-miR-206 1
GSM785479 pre-miR-27b 1
BioProject PRJNA145531

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31620_RAW.tar 36.6 Mb (http)(custom) TAR (of CEL, GPR)
Processed data included within Sample table

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