|
| Status |
Public on Oct 13, 2011 |
| Title |
The Dynamic Architecture of Hox Gene Clusters |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The spatial and temporal control of Hox gene transcription is essential for patterning the vertebrate body axis. Although this process involves changes in histone posttranslational modifications, the existence of particular three-dimensional (3D) architectures remained to be assessed in vivo. Using high-resolution chromatin conformation capture methodology, we examined the spatial configuration of Hox clusters in embryonic mouse tissues where different Hox genes are active. When the cluster is transcriptionally inactive, Hox genes associate into a single 3D structure delimited from flanking regions. Once transcription starts, Hox clusters switch to a bimodal 3D organization where newly activated genes progressively cluster into a transcriptionally active compartment. This transition in spatial configurations coincides with the dynamics of chromatin marks, which label the progression of the gene clusters from a negative to a positive transcription status. This spatial compartmentalization may be key to process the collinear activation of these compact gene clusters.
|
| |
|
| Overall design |
Examination of gene expression in 3 cell types. Examination of 2 different histone modifications in 2 cell types.
|
| |
|
| Contributor(s) |
Noordermeer D, Leleu M, Duboule D |
| Citation(s) |
21998387, 24843030 |
| Submission date |
Aug 22, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Marion LELEU |
| Organization name |
EPFL
|
| Department |
School of Life Sciences
|
| Lab |
Laboratory of developmental genomics
|
| Street address |
EPFL-SV-ISREC-UPDUB- Station 19
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (11)
|
| GSM783825 |
anterior trunk expression replicate1 |
| GSM783826 |
anterior trunk expression replicate2 |
| GSM783827 |
forebrain expression replicate1 |
|
| Relations |
| SRA |
SRP007952 |
| BioProject |
PRJNA145705 |
Less...
More...