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Series GSE31328 Query DataSets for GSE31328
Status Public on Aug 14, 2011
Title A genome-wide expression profile of steroidogenic cells selectively isolated from adrenal glands of knockout mice lacking steroidogenic acute regulatory protein
Organism Mus musculus
Experiment type Expression profiling by array
Summary Steroidogenic acute regulatory protein (Star) facilitates cholesterol transfer into the inner mitochondrial membrane in the acute and regulated production of steroid hormones. Mice lacking Star (Star-/-) share the phenotypes with patients with congenital lipoid adrenal hyperplasia such as compromised production of steroid hormones and florid accumulation of cholesterol esters in adrenal glands and gonads. To define specific patterns of molecular changes with disruption of Star, we performed a transcriptome analysis of steroidogenic cells in adrenal glands. We harvested adrenal glands at E17.5 or 18.5 from seven wild-type (Star+/+) mice or four Star-/- mice having the transgene targeting enhanced green fluorescent protein (eGFP) under the control of regulatory sequences of mouse Star gene. Steroidogenic cells were selectively isolated by fluorescent-activated cell sorting. The gene expression profile of the fluorescence-positive cells was obtained with Agilent Whole Mouse Genome Microarray and was confirmed by quantitative real-time PCR. We identified 961 and 622 genes that were significantly up-regulated and down-regulated, respectively, in Star-/- mice compared with Star+/+ mice (fold difference ≥2, p value of Student t test <0.05, and Benjamini-Hochberg false discovery rate of multiple comparison test <0.2). In Star-/- mice, expression levels of genes involved in cholesterol mobilization and efflux or immune response as antigen presenting cells were significantly increased, and transition from fetal to adult adrenocortical cells were significantly decreased, whereas those of genes related to steroid hormone biosynthesis or cholesterol biosynthesis and influx were not significantly changed. The trancriptome analysis revealed hitherto undescribed characteristic changes in adrenocortical cells and expanded our understanding of the pathophysiology of the steroidogenic cells with disruption of Star.
Overall design Gene expression profiles of isolated adrenal steroidogenic cells were compared between seven wild-type mice and four knockout mice lacking steroidogenic acute regulatory protein at E17.5-18.5.
Contributor(s) Ishii T, Mitsui T, Suzuki S, Matsuzaki Y, Parker KL, Hasegawa T
Citation(s) 22529212
Submission date Aug 10, 2011
Last update date Jan 12, 2017
Contact name Tomohiro Ishii
Organization name School of Medicine, Keio University
Department Pediatrics
Lab Endocrinology and metabolism
Street address 35 Shinanomachi, Shinjuku-ku
City Tokyo
ZIP/Postal code 160-8582
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (11)
GSM777008 Adrenocortical cell_wild-type_replicate 1
GSM777009 Adrenocortical cell_wild-type_replicate 2
GSM777010 Adrenocortical cell_wild-type_replicate 3
BioProject PRJNA145995

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GSE31328_RAW.tar 22.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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