GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE31092 Query DataSets for GSE31092
Status Public on Aug 02, 2011
Title Expression analysis of ZBP-89 deficient human primary erythroid progenitors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The molecular mechanisms underlying erythroid-specific gene regulation remain incompletely understood. Closely spaced binding sites for GATA, NF-E2/maf and CACCC interacting transcription factors play functionally important roles in globin and other erythroid-specific gene expression. We and others recently identified the CACCC-binding transcription factor ZBP-89 as a novel GATA-1 and NF-E2/mafK interacting partner. Here, we examined the role of ZBP-89 in human globin gene regulation and erythroid maturation using a primary CD34+ cell ex vivo differentiation system. We show that ZBP-89 protein levels rise dramatically during human erythroid differentiation, and that ZBP-89 occupies key cis-regulatory elements within the globin and other erythroid gene loci. ZBP-89 binding correlates strongly with RNA Pol II occupancy, active histone marks, and high-level gene expression. ZBP-89 physically associates with the histone acetyltransferases (HATs) p300 and Gcn5/Trrap, and occupies common sites with Gcn5 within the human globin loci. Lentiviral shRNA knockdown of ZBP-89 results in reduced Gcn5 occupancy, decreased acetylated histone 3 levels, lower globin and erythroid-specific gene expression, and impaired erythroid maturation. Addition of the HDAC inhibitor valproic acid partially reverses the reduced globin gene expression. These findings reveal an activating role for ZBP-89 in human globin gene regulation and erythroid differentiation.
Keywords: Human primary erythroid progenitors
Overall design Expression data of human erythroid progenitors transduced with lentivirus expressing short hairpins against ZBP-89 and control empty vector
Contributor(s) Woo AJ, Kim J, Xu J, Huang H, Cantor AB
Citation(s) 21828133
Submission date Aug 01, 2011
Last update date Dec 06, 2018
Contact name Andrew Jonghan Woo
Phone 6179192028
Fax 6177300222
Organization name Children's Hospital Boston / Harvard Medical School
Department Hematology / Oncology
Lab Alan B Cantor Lab
Street address 1 Blackfan Circle, Karp Res Bldg
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM769889 Empty vector, biological rep1
GSM769890 Empty vector, biological rep2
GSM769891 shZBP-89, biological rep1
BioProject PRJNA144709

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31092_RAW.tar 9.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap