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Series GSE30859 Query DataSets for GSE30859
Status Public on Sep 01, 2011
Title Multilineage Priming of Enhancer Repertoires Precedes Commitment to the B and Myeloid Cell Lineages in Hematopoietic Progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Genome binding/occupancy profiling by high throughput sequencing
Summary Recent studies have documented genome-wide binding patterns of transcriptional regulators and their associated epigenetic marks in hematopoietic cell lineages. In order to determine how epigenetic marks are established and maintained during developmental progression, we have generated long-term cultures of hematopoietic progenitors by enforcing the expression of the E-protein antagonist Id2. Hematopoietic progenitors that express Id2 are multipotent and readily differentiate upon withdrawal of Id2 expression into committed B lineage cells, thus indicating a causative role for E2A (Tcf3) in promoting the B cell fate. Genome-wide analyses revealed that a substantial fraction of lymphoid and myeloid enhancers are premarked by the poised or active enhancer mark H3K4me1 in multipotent progenitors. Thus, in hematopoietic progenitors, multilineage priming of enhancer elements precedes commitment to the lymphoid or myeloid cell lineages.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Citation(s) 21903424
Submission date Jul 21, 2011
Last update date May 15, 2019
Contact name Yin Chun Lin
E-mail(s) yclin@ucsd.edu
Organization name UCSD
Department Division of Biological Sciences
Lab Cornelis Murre
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093-0377
Country USA
 
Platforms (5)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL6105 Illumina mouse-6 v1.1 expression beadchip
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (35)
GSM765549 Uninduced Id2-HPC, biological replicate 1
GSM765550 Uninduced Id2-HPC, biological replicate 2
GSM765551 Cultured E2A-deficient, biological replicate 1
This SuperSeries is composed of the following SubSeries:
GSE30855 Establishment of Enhancer Repertoires that Orchestrate the Myeloid and Lymphoid Cell Fates (gene expression dataset 1)
GSE30856 Establishment of Enhancer Repertoires that Orchestrate the Myeloid and Lymphoid Cell Fates (gene expression dataset 2)
GSE30858 Establishment of Enhancer Repertoires that Orchestrate the Myeloid and Lymphoid Cell Fates (ChIP-Seq dataset)
Relations
BioProject PRJNA146145

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30859_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, CEL)
SRA Run SelectorHelp
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