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Status |
Public on Jul 19, 2011 |
Title |
Enrichment profiles of Ser-5 phosphorylated RNA polymerase II (PolII S5p) in mouse female ES cells |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
Many animal species employ a chromosome-based mechanism of sex determination, which has led to coordinate evolution of dosage compensation systems. Dosage compensation not only corrects the imbalance in the number of X-chromosomes between the sexes, but is also hypothesized to correct dosage imbalance within cells due to mono-allelic X expression and bi-allelic autosomal expression, by upregulating X-linked genes (termed ‘Ohno’s hypothesis’). It is unknown whether any epigenetic mark or protein is involved in X upregulation in mammals. Ser-5 phosphorylated RNA polymerase II (PolII S5p) is required for transcription initiation. Chromatin immunoprecipitation combined with DNA tiling array analysis (ChIP-chip) of PolII S5p in mouse female ES cells with two active X chromosomes demonstrated a greater enrichment of RNA polymerase II on X-linked genes relative to autosomal genes, suggesting that enhanced transcription initiation may play a role in X upregulation.
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Overall design |
Comparison of RNA PolII S5p enrichment on the X versus autosomes in mouse
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Contributor(s) |
Deng X, Disteche CM |
Citation(s) |
22019781, 23523075, 26248554 |
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Submission date |
Jul 14, 2011 |
Last update date |
Oct 09, 2019 |
Contact name |
Xinxian Deng |
E-mail(s) |
dengx2@u.washington.edu
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Organization name |
University of Washington
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Department |
Laboratory Medicine and Pathology
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Lab |
HSB C526
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Street address |
1959 NE Pacific St.
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City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98195 |
Country |
USA |
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Platforms (1) |
GPL9833 |
NimbleGen Mus musculus 2.1M 2007-09-21_MM8_Deluxe_Promoter_HX1 tiling design |
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Samples (2) |
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This SubSeries is part of SuperSeries: |
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Relations |
BioProject |
PRJNA154693 |