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| Status |
Public on Dec 05, 2005 |
| Title |
Gene expression responses to a Salmonella infection in the chicken intestine differ between lines. |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
Poultry products are an important source of Salmonella enterica. An effective way to reduce food poisoning due to Salmonella would be to breed chickens more resistant to Salmonella. Unfortunately resistance to Salmonella is a complex trait with many factors involved. To learn more about Salmonella resistance mechanisms in young chickens, a cDNA microarray analysis was performed to compare gene expression profiles between a Salmonella susceptible and a more resistant chicken line. Newly hatched chickens were orally infected with S. enterica serovar Enteritidis. Since the intestine is the first barrier the bacteria encountersbacteria encounter after oral inoculation, gene expression was investigated in the intestine, from day 1 until day 21 post infection. Differences in gene expression between the susceptible and resistant chicken line were found in control as well as Salmonella infected conditions. In response to the Salmonella infection, the expression of different sets of genes seemed to be affected in the jejunum of the two chicken lines. In the susceptible line this included genes that affect T-cell activation, whereas in the more resistant line, at day 1, macrophage activation seemed to be more affected. At day 7 and 9 most gene expression differences between the two chicken lines were identified under control conditions, indicating a difference in the intestinal development between the two chicken lines which might be linked to the difference in Salmonella susceptibility. The findings in this study have lead to the identification of novel genes and possible cellular pathways of the host involved in Salmonella susceptibility.
Keywords: timecourse, disease
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| Overall design |
8 timepoints with four groups for each timepoints. Pooled samples of 5 animals for each group. The different groups are:
slow growing control
slow growing salmonella
fast growing control
fast growing salmonella
Four comparisions for each timepoint: slow control- slow salmonella, fast control-fast salmonella, slow control-fast control, slow salmonella-fast salmonella. A dye-swap was done, so 8 slides for each timepoints, 64 arrays in total.
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| Contributor(s) |
van Hemert S, Hoekman AJ, Smits MA, Rebel JM |
| Citation(s) |
16978708 |
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| Submission date |
Aug 05, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Saskia van Hemert |
| E-mail(s) |
saskia.vanhemert@wur.nl
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| Organization name |
Animal Sciences Group
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| Street address |
PO box 65
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| City |
Lelystad |
| ZIP/Postal code |
8200 AB |
| Country |
Netherlands |
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| Platforms (1) |
| GPL2719 |
intestine-spleen combined array |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA93159 |
| Supplementary data files not provided |
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