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Series GSE30504 Query DataSets for GSE30504
Status Public on Sep 21, 2011
Title Whole Genome Expression in Peripheral-Blood Samples of Workers Professionally Exposed to Polycyclic Aromatic Hydrocarbons
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This study aims to examine global gene expression profiles before and after the work-shift among coke-oven workers (COW). COW work six consecutive days and then take two days off. Two blood and urine samples in each worker were collected before starting to work after two-days off and end-of-shift in the sixth-day work in 2009. Altered gene expressions (ratio of gene expression levels between end-of-shift and pre-shift work) were performed by Human OneArray expression system which probes ~30,000-transcription expression profiling of human genes. Sixteen workers, all men, were enrolled in this study. Median urinary 1-hydroxypyrene (1OHP) levels (umole/mole creatinine) in end-of-shift work were significantly higher than those in pre-shift work (2.58 vs. 0.29, p = 0.0002). Among the 20,341 genes which passed experimental quality control, 26 gene expression changes, 7 positively- and 19 negatively-, were highly correlated with across-the-shift urinary 1OHP levels (end-of-shift – pre-shift 1OHP) (p-value < 0.001). The high and low exposure groups of across-the-shift urinary 1OHP levels dichotomized in ~2.00 µmole/mole creatinine were able to be distinguished by these 26 genes. Some of them are known to be involved in apoptosis, chromosome stability/DNA repair, cell cycle control/tumor suppressor, cell adhesion, development/spermatogenesis, immune function, and neuronal cell function.
Overall design Sixteen coke-oven workers who had worked in one of two coke-oven plants for at least one year in the largest steel company in Taiwan voluntarily participated in this study between July-October, 2009. Coke-oven workers regularly work 6 days and take two days off. Thus, we collected their blood and urine samples at two different time points: one was during the pre-shift work on the first day after two days off, and the second one was at the end-of-shift work on the 6th work day. Information about age and smoking status was also collected before the collection of biological specimens.
Contributor(s) Wu M, Pan C, Su H, Huang J, Peng C, Wang W, Chou T, Lin M, Lin W, Huang C, Lee T, Ho C
Citation(s) 21854004
Submission date Jul 08, 2011
Last update date Aug 28, 2014
Contact name Tzu Chi Lee
Organization name Kaohsiung Medical University
Department Department of Public Health
Street address Shihcyuan 1st Rd.
City Kaohsiung
ZIP/Postal code 80708
Country Taiwan
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (32)
GSM756580 Patient 1 Preshift
GSM756581 Patient 1 End-of-shift
GSM756582 Patient 2 Preshift
BioProject PRJNA143317

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30504_RAW.tar 206.7 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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