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Series GSE30198 Query DataSets for GSE30198
Status Public on Jun 25, 2011
Title Digital gene expression and global mapping of polyadenylation sites with PolyA-Seq
Organisms Macaca mulatta; Homo sapiens; Canis lupus familiaris; Mus musculus; Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary We describe PolyA-Seq, a strand-specific method for high-throughput sequencing of the 3' ends of polyadenylated transcripts. PolyA-Seq is as accurate for digital gene expression as existing RNA sequencing approaches, and superior to microarrays. We used the approach to map polyadenylation (polyA) sites in 24 samples from normal tissues in human, rhesus, dog, mouse, and rat.
Overall design Detection of polyA sites in a mixture of 24 tissues in human, mouse, rat, dog and rhesus. Samples included two replicates each of MAQC Human Brain Reference and MAQC Universal Human Reference. Two additional human sets of reads are included that were used to distinguish true polyA sites from internal priming sites.
Contributor(s) Derti A, Babak T
Citation(s) 22454233
Submission date Jun 24, 2011
Last update date May 15, 2019
Contact name Adnan Derti
Organization name personal
Department Bioinformatics
Street address 100 Alden Street
City Dedham
State/province MA
ZIP/Postal code 02026
Country USA
Platforms (5)
GPL10669 Illumina Genome Analyzer IIx (Rattus norvegicus)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (26)
GSM747467 dog_brain
GSM747468 dog_kidney
GSM747469 dog_testis
SRA SRP007359
BioProject PRJNA143955

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30198_RAW.tar 294.7 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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