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Series GSE30190 Query DataSets for GSE30190
Status Public on Dec 22, 2011
Title Comparison of gene expression between Arx-transfected N2a cells and cells transfected by the corresponding empty vector
Organism Mus musculus
Experiment type Expression profiling by array
Summary Genetic investigations of X-linked intellectual disabilities have implicated the ARX (Aristaless-related homeobox) gene in a wide spectrum of disorders extending from phenotypes characterised by severe neuronal migration defects such as lissencephaly, to mild or moderate forms of mental retardation without apparent brain abnormalities but with associated features of dystonia and epilepsy. Analysis of Arx spatio-temporal localisation profile in mouse revealed expression in telencephalic structures, mainly restricted to populations of GABAergic neurons at all stages of development. Furthermore, studies of the effects of ARX loss of function in humans and animal models revealed varying defects, suggesting multiple roles of this gene during brain development. However, to date, little is known about how ARX functions as a transcription factor and the nature of its targets. To better understand its role, we combined chromatin immunoprecipitation and mRNA expression with microarray analysis and identified a total of 1006 gene promoters bound by Arx in transfected neuroblastoma (N2a) cells and in mouse embryonic brain. Some of these promoters were enriched for a sequence very similar to a motif previously identified as Arx-binding motif and approximately 24% of Arx-bound genes were found to show expression changes following Arx overexpression or knock-down. Several of the Arx target genes we identified are known to be important for a variety of functions in brain development, including axonal guidance and synaptic plasticity and some of them suggest new functions for Arx. Overall, these results identified multiple new candidate targets for Arx and should help to better understand the pathophysiological mechanisms of intellectual disability and epilepsy associated with ARX mutations.
Overall design N2a cells were transfected with either Arx or the corresponding empty vector. Eight different independent experiments were performed. The 16 samples were randomly distributes on the 2 expression microarrays.
Contributor(s) Quillé M, Hirchaud E, Carat S, Baron D, Houlgatte R, Friocourt G
Citation(s) 21966449
Submission date Jun 24, 2011
Last update date Jan 19, 2018
Contact name Gaelle Friocourt
Phone +33 298443895
Fax +33 298467910
Organization name Inserm U613
Department Molecular Genetics
Street address 46 rue Félix Le Dantec, CS51819
City Brest
ZIP/Postal code 29218
Country France
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (16)
GSM747388 N2a cells transfected by FLAG 1
GSM747389 N2a cells transfected by FLAG 2
GSM747390 N2a cells transfected by FLAG 3
This SubSeries is part of SuperSeries:
GSE30191 ARX transcription factor implicated in mental retardation and epilepsy
BioProject PRJNA155105

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30190_RAW.tar 73.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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