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Status |
Public on Oct 17, 2012 |
Title |
FGF inhibition directs BMP4-mediated differentiation of Human Embryonic Stem Cells to syncytiotrophoblast |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Bone morphogenetic protein (BMP) signaling is known to support differentiation of human embryonic stem cells (hESCs) into mesoderm and extraembryonic lineages, whereas other signaling pathways can largely influence this lineage specification. Here, we set out to reinvestigate the influence of ACTIVIN/NODAL and fibroblast growth factor (FGF) pathways on the lineage choices made by hESCs during BMP4-driven differentiation. We show that BMP activation, coupled with inhibition of both ACTIVIN/NODAL and FGF signaling, induces differentiation of hESCs, specifically to βhCG hormone-secreting multinucleated syncytiotrophoblast and does not support induction of embryonic and extraembryonic lineages, extravillous trophoblast, and primitive endoderm. It has been previously reported that FGF2 can switch BMP4-induced hESC differentiation outcome to mesendoderm. Here, we show that FGF inhibition alone, or in combination with either ACTIVIN/NODAL inhibition or BMP activation, supports hESC differentiation to hCG-secreting syncytiotrophoblast. We show that the inhibition of the FGF pathway acts as a key in directing BMP4-mediated hESC differentiation to syncytiotrophoblast.
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Overall design |
Human embryonic Stem Cells (hESCs) were treated under defined conditions (N2B27) with BMP4 (B), SB431542 (SB) (ACTIVIN/NODAL inhibitor), SU5402 (SU) (FGFR1 inhibitor), FGF2 (F) either alone or in various combinations as mentioned, followed by isolation of total RNA.
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Contributor(s) |
Sudheer S, Bhushan R, Fauler B, Lehrach H, Adjaye J |
Citation(s) |
22724507 |
Submission date |
Jun 21, 2011 |
Last update date |
Mar 20, 2017 |
Contact name |
smita sudheer |
E-mail(s) |
sudheer@molgen.mpg.de
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Organization name |
Max Planck Institute for Molecular Genetics
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Street address |
Ihnestraße 63-73
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City |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
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Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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Samples (24)
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Relations |
BioProject |
PRJNA144037 |
Supplementary file |
Size |
Download |
File type/resource |
GSE30125_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR |
GSE30125_non-normalized.txt.gz |
4.4 Mb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
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