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Series GSE30114 Query DataSets for GSE30114
Status Public on May 10, 2012
Title Microarray analysis of gene expression differences in prostate specific antigen negative (PSA-ve) cells versus PSA+ve cells in LAPC9 and LNCaP prostate cancer (PCa) cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Prostate cancer (PCa) is heterogeneous containing both phenotypically differentiated and undifferentiated tumor cells. An important unanswered question is whether these two populations of PCa cells are functionally different. Here we report the distinct molecular, cellular, and tumor-propagating properties of PCa cells that express high (i.e., PSA+) and low (PSA-/lo) levels of the differentiation marker PSA (prostatespecific antigen). PSA-/lo PCa cells are quiescent and resistant to multiple stresses including androgen deprivation, exhibit high clonogenic potential, and possess long-term tumor-propagating capacity in male mice. They preferentially express stem cell-associated genes and can undergo asymmetric cell division generating PSA+ cells. Importantly, PSA-/lo PCa cells can initiate robust tumor development in castrated hosts, survive androgen deprivation, and harbor highly tumorigenic castration-resistant PCa cells that can be further enriched using the ALDH+CD44+α2β1+ phenotype. In contrast, PSA+ PCa cells possess more limited tumor-propagating capacity, mainly undergo symmetric division, and are sensitive to castration. Together, our study suggests that PSA-/lo and PSA+ PCa cells are functionally distinct and PSA-/lo cells may represent one critical source of castration-resistant PCa cells.
 
Overall design There are two sets of samples in this study corresponding to two different prostate cancer cell lines LNCaP and LAPC9. Both LNCaP and LAPC9 sets include 3 technical triplicates each that were performed onvdual color arrays and each individual array includes comparison between PSA- (Cy5) and PSA+ (Cy3) cells of the respective cell line.
 
Contributor(s) Qin J, Liu X, Laffin B, Chen X, Choy G, Jeter C, Calhoun-Davis T, Li H, Palapattu GS, Pang S, Lin K, Huang J, Ivanov I, Suraneni MV, Tang DG
Citation(s) 26246472
Submission date Jun 20, 2011
Last update date Jul 30, 2019
Contact name Dean G Tang
E-mail(s) dtang@mdanderson.org
Organization name U.T M. D. Anderson Cancer Center
Department Carcinogenesis
Lab Dean G Tang's Lab
Street address 1808 Park road 1C
City Smithville
State/province TX
ZIP/Postal code 78957
Country USA
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (6)
GSM386636 LAPC9 PSA-vsPSA+_rep1
GSM386637 LAPC9 PSA-vsPSA+_rep2
GSM386826 LAPC9 PSA-vsPSA+_rep3
This SubSeries is part of SuperSeries:
GSE35814 The PSA-/lo prostate cancer cells harbor self-renewing long-term tumor-propagating cells that resist castration
Relations
BioProject PRJNA155491

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30114_RAW.tar 92.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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