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Series GSE29917 Query DataSets for GSE29917
Status Public on Nov 21, 2011
Title 17beta-estradiol (E2)-regulated gene expression across time in estrogen-dependent and estrogen deprivation-resistant MCF-7-derived breast cancer cell lines.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A series of MCF-7 variants were previously developed that are either estrogen-dependent for growth (MCF-7:WS8 cells), or resistant to estrogen deprivation and refractory (MCF-7:2A) or sensitive (MCF-7:5C) to E2-induced apoptosis.
To identify genes associated with E2-induced apoptosis, estrogen deprivation-resistant/apoptotic-sensitive 5C cells were compared to both estrogen-dependent MCF-7:WS8 and estrogen deprivation/apoptotic-refractory MCF-7:2A cells
Overall design Each cell line was treated with 10-9 M E2 or vehicle control over a 96 h time course consisting of 7 time points (2, 6, 12, 24, 48, 72 and 96 h) using 6 biological replicates per condition. cRNA probes from individual E2-treated samples were competitively hybridized against time-matched pooled control probes using 2-color Agilent 4x44k human oligonucleotide microarrays.
Contributor(s) Ariazi EA, Cunliffe HE, Ramos P, Willis AL, Slifker MJ, Jordan VC
Citation(s) 22011582
Submission date Jun 13, 2011
Last update date Feb 22, 2018
Contact name Michael Slifker
Organization name Fox Chase Cancer Center
Street address 333 Cottman Ave.
City Philadelphia
State/province PA
ZIP/Postal code 19111
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (118)
GSM740721 WS8 2hr Replicate 1
GSM740722 WS8 2hr Replicate 3
GSM740723 WS8 2hr Replicate 4
BioProject PRJNA140715

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29917_RAW.tar 1.8 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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