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Series GSE29913 Query DataSets for GSE29913
Status Public on Jan 20, 2012
Title Genome-wide distribution maps of histone variants H2A.Lap1 and H2A.Z in 30do mouse spermatids [ChIP_seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromatin performs numerous functions during cellular differentiation, and therefore it must be capable of adopting a multitude of different structures. How these various structures are established is poorly understood, but we propose that specific histone H2A variants will have a key role in remodelling chromatin during differentiation. Structurally, we show here that the gain of just a single acidic amino acid residue has generated a new mouse H2A.Bbd-like histone variant, H2A.Lap1, and that when incorporated into nucleosomal arrays imparts on them unique biophysical properties that are distinct from arrays containing either H2A or human H2A.Bbd. Functionally, we identify H2A.Lap1 as a novel chromatin component of active genes that are expressed during spermatogenesis, and in combination with H2A.Z create a unique chromatin landscape at the start site of transcription. During round spermatid differentiation, H2A.Lap1 dramatically loads onto the inactive X chromosome enabling a subset of its genes to be transcriptionally activated
Overall design Examination of 2 different histone variants in mouse spermatids.
Contributor(s) Huttley G, Pahwa A, Nekrasov M
Citation(s) 22139013
Submission date Jun 13, 2011
Last update date May 15, 2019
Contact name Rohan Williams
Organization name ANU
Street address Garran Rd ACTON
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM740673 H2A.Lap1_ChIPSeq
GSM740674 H2A.Lap1-2_ChIPSeq
GSM740675 H2A.Z_ChIPSeq
This SubSeries is part of SuperSeries:
GSE29916 Functional studies of a H2A.Bbd-like histone variant in mouse spermatogenesis
SRA SRP007115
BioProject PRJNA155327

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29913_RAW.tar 179.8 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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