|
| Status |
Public on Jan 20, 2012 |
| Title |
Genome-wide distribution maps of histone variants H2A.Lap1 and H2A.Z in 30do mouse spermatids [ChIP_seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin performs numerous functions during cellular differentiation, and therefore it must be capable of adopting a multitude of different structures. How these various structures are established is poorly understood, but we propose that specific histone H2A variants will have a key role in remodelling chromatin during differentiation. Structurally, we show here that the gain of just a single acidic amino acid residue has generated a new mouse H2A.Bbd-like histone variant, H2A.Lap1, and that when incorporated into nucleosomal arrays imparts on them unique biophysical properties that are distinct from arrays containing either H2A or human H2A.Bbd. Functionally, we identify H2A.Lap1 as a novel chromatin component of active genes that are expressed during spermatogenesis, and in combination with H2A.Z create a unique chromatin landscape at the start site of transcription. During round spermatid differentiation, H2A.Lap1 dramatically loads onto the inactive X chromosome enabling a subset of its genes to be transcriptionally activated
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| |
|
| Overall design |
Examination of 2 different histone variants in mouse spermatids.
|
| |
|
| Contributor(s) |
Huttley G, Pahwa A, Nekrasov M |
| Citation(s) |
22139013 |
| |
| Submission date |
Jun 13, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Rohan Williams |
| E-mail(s) |
rohan.williams@anu.edu.au
|
| Organization name |
ANU
|
| Street address |
Garran Rd ACTON
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE29916 |
Functional studies of a H2A.Bbd-like histone variant in mouse spermatogenesis |
|
| Relations |
| SRA |
SRP007115 |
| BioProject |
PRJNA155327 |
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