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| Status |
Public on Nov 01, 2011 |
| Title |
The transcriptome of the arbuscular mycorrhizal fungus Glomus intraradices |
| Platform organism |
Rhizophagus intraradices |
| Sample organisms |
Medicago truncatula; Oryza sativa; Rhizophagus intraradices |
| Experiment type |
Expression profiling by array
|
| Summary |
The virtual transcriptome of G. intraradices (based on >430,000 reads) served as the basis for the design of an EST expresson array and for a number of analyses of gene expression in germinating spores, extraradical mycelium (ERM) and symbiotic root tissues.
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| Overall design |
The G. intraradices EST expression array (4 x 72K) manufactured by Roche NimbleGen Systems Limited (Madison, WI) (http://www.nimblegen.com/products/exp/index.html) contained three independent, non identical, 60-mer probes per sequence. Included in the oligoarray were 22,402 G. intraradices sequences, 5785 random 60-mer control probes and labeling controls. We performed 12 hybridisations with samples from germinating spores (three biological replicates), extraradical mycelium (three biological replicates), symbiotic root tissues from Medicago (three biological replicates) and rice (1 replicate) as well as from microdissected arbuscule-colonized cortical cells of Medicago and rice (1 replicate each).
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| Contributor(s) |
Kohler A, Tisserant E, Martin F, Balestrini R, Gomez SK, Ferrol N, Paszkowski U, Roux C, Harrisson M, Seddas P |
| Citation missing |
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| |
| Submission date |
Jun 09, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
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| Platforms (1) |
| GPL13705 |
Glomus intraradices EST expression array |
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| Samples (12)
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| GSM739632 |
Extraradical mycelium 1 |
| GSM739633 |
Extraradical mycelium 2 |
| GSM739635 |
Extraradical mycelium 3 |
| GSM739636 |
G. intraradices-inoculated roots of M. truncatula 1 |
| GSM739639 |
G. intraradices-inoculated roots of M. truncatula 2 |
| GSM739646 |
G. intraradices-inoculated roots of M. truncatula 3 |
| GSM739665 |
G. intraradices-inoculated roots of rice |
| GSM739711 |
Microdissected arbuscule-colonized cortical cells from Rice |
| GSM739786 |
Microdissected arbuscule-colonized cortical cells from Medicago |
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| Relations |
| BioProject |
PRJNA140979 |
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