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| Status |
Public on Aug 28, 2011 |
| Title |
Spo11-accessory proteins link DNA double-strand break sites to the chromosome axis in early meiotic recombination |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
Meiotic recombination between homologous chromosomes initiates via programmed DNA double-strand breaks (DSBs), generated by complexes comprising Spo11 transesterase plus accessory proteins. DSBs arise concomitantly with the development of axial chromosome structures, where the coalescence of axis sites produces linear arrays of chromatin loops. Recombining DNA sequences map to loops, but are ultimately tethered to the underlying axis. How and when such tethering occurs is currently unclear. Using ChIPchip in yeast, we show that Spo11-accessory proteins Rec114, Mer2 and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S-phase Cdk. This axis tethering requires meiotic axis components (Red1/Hop1) and is modulated in a domain-specific fashion by cohesin. Loss of Rec114, Mer2 and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hotspot sequences become tethered to axis sites by the DSB machinery prior to DSB formation.
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| Overall design |
34 ChIPs
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| Contributor(s) |
Panizza S, Mendoza MA, Berlinger M, Huang L, Nicolas A, Shirahige K, Klein F |
| Citation(s) |
21816273 |
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| Submission date |
Jun 09, 2011 |
| Last update date |
Jan 09, 2016 |
| Contact name |
Franz Klein |
| E-mail(s) |
franz.klein@univie.ac.at
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| Phone |
++431427756220
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| Organization name |
University of Vienna, MFPL
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| Department |
Chromosome Biology
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| Street address |
Dr. Bohrg. 9
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| City |
Vienna |
| ZIP/Postal code |
A-1030 |
| Country |
Austria |
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| Platforms (2) |
| GPL7249 |
[Sc03b_MF] Affymetrix GeneChip S. cerevisiae Tiling 1.0F Array |
| GPL7250 |
[Sc03b_MR] Affymetrix GeneChip S. cerevisiae Tiling 1.0R Array |
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| Samples (34)
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| GSM739671 |
Red1, 4h meiosis |
| GSM739672 |
Mer2, 4h meiosis, biological repeat 1 |
| GSM739673 |
Rec114, 4h meiosis, biological repeat 1 |
| GSM739674 |
Mei4, 4h meiosis |
| GSM739675 |
Rec114, 4h meiosis, biological repeat 2 |
| GSM739676 |
Rec104, 4h meiosis, biological repeat 1 |
| GSM739677 |
Rec114, 3h meiosis |
| GSM739678 |
Rec114, mer2delta mutant 3h meiosis |
| GSM739679 |
Rec114, mei4delta mutant 3h meiosis |
| GSM739680 |
Rec114, spo11-Y135f mutant 4h meiosis |
| GSM739681 |
Rec114, rec104delta mutant 3h meiosis |
| GSM739682 |
Rec114, clb5delta clb6delta double-mutant 4h meiosis |
| GSM739683 |
Mer2 rec114delta, 4h meiosis, biological repeat 1 |
| GSM739684 |
Mer2 mei4delta, 3h meiosis |
| GSM739685 |
Mer2 red1delta, 4h meiosis, biological repeat 1 |
| GSM739686 |
Mer2 clb5delta clb6delta double-mutant 4h meiosis, biological repeat 1 |
| GSM739687 |
mer2-S30A , 4h meiosis |
| GSM739688 |
Rec114 hop1delta mutant, 4h meiosis |
| GSM739689 |
Rec114 red1delta mutant, 4h meiosis |
| GSM739690 |
Rec114 rec8delta mutant, 4h meiosis, biological repeat 1 |
| GSM739691 |
Hop1 rec8delta mutant, 4h meiosis, biological repeat 1 |
| GSM739692 |
Rec104, 4h meiosis, biological repeat 2 |
| GSM739693 |
Mer2, 4h meiosis, biological repeat 2 |
| GSM739694 |
Mer2 rec114delta, 4h meiosis, biological repeat 2 |
| GSM739695 |
Mer2 hop1delta, 4h meiosis |
| GSM739696 |
Mer2 red1delta, 4h meiosis, biological repeat 2 |
| GSM739697 |
Mer2, 3h meiosis |
| GSM739698 |
mer2-S30A , 3h meiosis |
| GSM739699 |
Mer2 clb5delta clb6delta double-mutant 4h meiosis, biological repeat 2 |
| GSM739700 |
Rec114 rec8delta mutant, 4h meiosis, biological repeat 2 |
| GSM739701 |
Hop1 rec8delta mutant, 4h meiosis, biological repeat 2 |
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| Relations |
| BioProject |
PRJNA140909 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29860_RAW.tar |
2.6 Gb |
(http)(custom) |
TAR (of CEL, TXT) |
| Processed data provided as supplementary file |
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