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| Status |
Public on Jul 04, 2011 |
| Title |
Role of miR-30 miRNAs during adipogenesis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of human adipose tissue-derived stem cells (hMADS cells) overexpressing either miR-30a or miR-30d, as well as knocked-down for the whole miR-30 family. Gene expression was analyzed after 4 days in adipogenic medium. Data give insight into the role of miR-30 during adipogenesis, and identifies enriched predicted targets for miR-30.
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| Overall design |
Human adipose tissue-derived stem cells (hMADS cells) were transfected with one of the following: premiR-30a (Ambion), premiR-30d (Ambion), premiR negative control #1 (Ambion). Three days after transfection, cells were submitted to adipogenic differentiation for 4 days. In a separate experiment, hMADS cells were transfected with either antimiR-30 or antimiR-neg (mismatch control), and then submitted to differentiation as described above. For each experimental condition, 2 hMADS cell clones were used independently (clone B7 and B9).
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| Contributor(s) |
Zaragosi LE, Wdzieknoski B, Lebrigand K, Villageois P, Mari B, Dani C, Barbry P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 10, 2011 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
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| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
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| Street address |
660 route des lucioles
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| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA140053 |
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