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Series GSE28877 Query DataSets for GSE28877
Status Public on Apr 27, 2011
Title Differentially expressed genes in metallopanstimulin-1 knockdown cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify the differentially expressed genes in metallopanstimulin-1 (MPS-1) knockdown gastric cancer cells compared with negative control ones, we employed the microarray profiling analysis. MPS-1 was knockdown by retroviral interfering system in human gastric adenocarcinoma originated cell SGC7901 and the transfectants named P4, while the negative control named NC. Genes with greater than 1.5-fold change and P-value <0.05 were identified as differentially expressed genes between NC and P4 cells. Among those, apoptotic related gene (Gadd45β, cIAP2, Bcl2, CAD, Bid, etc) and adhesive related genes (integrin beta 4, ECM2, etc) were quantified by real-time PCR as well as western blotting.
Overall design The two groups of negative control (NC) and MPS-1 knockdown gastric cancer cells (P4) were harvested after puromycin screening. Three independent experiments were performed for each group.
Contributor(s) Yang Z, Gu Q
Citation(s) 21796632
Submission date Apr 27, 2011
Last update date Feb 22, 2018
Contact name Zhongyin Yang
Organization name Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
Department Department of Surgery
Street address NO.197 Ruijin er road
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (6)
GSM714815 Gastric cancer cells_negative control_1_rep1
GSM714816 Gastric cancer cells_negative control_1_rep2
GSM714817 Gastric cancer cells_negative control_1_rep3
BioProject PRJNA140599

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28877_RAW.tar 61.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Processed data provided as supplementary file

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