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Series GSE28696 Query DataSets for GSE28696
Status Public on Apr 19, 2011
Title Development of gene expression signatures on immunity for bursal peptide BPP-II
Organism Mus musculus
Experiment type Expression profiling by array
Summary To further elucidate the mechanism of BPP-II on immunity on the broad molecular level, we have employed whole genome microarray expression profiling as a discovery platform to examine gene expression patterns during BPP-II treatment in a mouse derived hybridoma culture system. Hybridoma cell was treated ex vivo, and robust normalization of the data identified 2478 differentially expressed probe sets exhibiting minimum 1.5-fold changes that distinguished between BPP-II and control samples. Various pathwayswere significantly impacted by BPP-II treatment, and gene Ontology annotations show changes in the expression of molecules involved in immune and immunocyte related cellular processes. Expression of 8 genes (MS4A2,CD86,CD80, Fas,CDKN2A,CBLC, FGF21 and Fyn) from this signature was quantified in the RNA samples by QRT-PCR, confirming low variability between the predicted response patterns.
Overall design BPP-II induced gene expression in hybridoma cell was measured at 4 hours after exposure to doses of 0ug/ml and 0.2ug/ml. Two independent experiments were performed using different cells for each experiment.
Contributor(s) Chen PY, Feng XL
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Submission date Apr 18, 2011
Last update date Jan 12, 2017
Contact name Pu Yan Chen
Organization name Nanjing Agriculture University
Street address No. 1, Weigang
City Nanjing
ZIP/Postal code 210095
Country China
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (2)
GSM675882 ExVivoHybridomacell_4hr_0ug/ml_rep1
GSM710704 ExVivoHybridomacell_4hr_0.2ug/ml_rep1
BioProject PRJNA138907

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28696_RAW.tar 4.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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