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Series GSE28688 Query DataSets for GSE28688
Status Public on Oct 25, 2011
Title Molecular insights into induced pluripotency mediated by the OCT4, SOX2, KLF and c-MYC gene regulatory network
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human somatic fibroblasts can be reprogrammed to induced pluripotent stem (iPS) cells by exogenic expression of the Yamanaka factors (OCT4, SOX2, KLF4 and MYC) after about 1 month. To gain some insight into the early processes operative in fibroblast reprogramming, we profiled genome-wide transcription levels using Illumina microarrays in the starting donor cells-human foreskin fibroblast (HFF1) cells and at three time points after OSKM transduction (24 h, 48 h, 72 h), as well as two iPS cell lines (iPS2, iPS4) and hES cell lines (H1, H9). We show that within the context of the viral transduction reprogramming protocol, the donor cell response to viral transfection perturbs redox homeostasis, which induces oxidative damage on the donor cells' protein and DNA. This leads to activation of p53, senescence, and apoptosis, greatly reducing the efficiency of reprogramming.
 
Overall design Total RNA obtained from HFF1 (human foreskin fibroblast) cells, OSKM-transduced HFF1 cells after 24h, 48h, 72h, undifferentiated hESCs, iPSCs.
 
Contributor(s) Ying W
Citation(s) 21909390
Submission date Apr 18, 2011
Last update date Mar 20, 2017
Contact name Ying Wang
E-mail(s) wang_y@molgen.mpg.de
Phone 00493084131237
Organization name Max Planck Institute for Molecular Genetics
Department Vertebrate Genomics
Street address Ihnestrasse 63
City Berlin
ZIP/Postal code 14195
Country Germany
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (14)
GSM710513 HFF1-a
GSM710514 HFF1-b
GSM710515 HFF1-24 h post-transduction-a
Relations
BioProject PRJNA139019

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28688_RAW.tar 3.9 Mb (http)(custom) TAR
GSE28688_non-normalized.txt.gz 1.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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