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Series GSE286034 Query DataSets for GSE286034
Status Public on Jan 14, 2025
Title Single-cell RNA sequencing identifies Atf3+ senescent chondrocytes in the aged rat meniscus
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary we conducted single-cell RNA sequencing (scRNA-seq) analysis using the 10x Chromium platform to uncover the cellular composition and molecular profiles of meniscal cells from different age groups.The meniscus, a fibrocartilaginous structure essential for joint load distribution, is highly susceptible to age-related degeneration. With aging, the meniscus experiences structural and functional deterioration, contributing to the progression of osteoarthritis(OA). This study aims to bridge this gap by profiling the cellular landscape and analyzing age-related changes in the aging meniscus using scRNA-seq.
 
Overall design we conducted single-cell RNA sequencing (scRNA-seq) analysis using the 10x Chromium platform to uncover the cellular composition and molecular profiles of meniscal cells from different age groups
Briefly, tissues were minced into ~1 mm³ pieces, digested with 4 mg/mL protease for 1 hour, followed by 0.25 mg/mL collagenase P for 4-6 hours. After washing with 0.04% bovine serum albumin (BSA) in phosphate-buffered saline (PBS), the cells were resuspended in 0.04% BSA and filtered through a 35 µm cell strainer. Viable cells were visually inspected, sorted via flow cytometry, and collected. Flow cytometry sorting ensured a viability threshold of >85% for single-cell RNA-seq
 
Contributor(s) Wang J, Liu Y, Pan Z, Liu Z, Tao K, Yin F
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Submission date Jan 07, 2025
Last update date Jan 14, 2025
Contact name Yaohui Liu
E-mail(s) liuyaohui22@tongji.edu.cn
Organization name Tongji University
Department School of Medicine
Street address 500 Zhennan Road,Putuo District
City shanghai
ZIP/Postal code 200331
Country China
 
Platforms (1)
GPL25947 Illumina NovaSeq 6000 (Rattus norvegicus)
Samples (2)
GSM8715381 AM
GSM8715382 YM
Relations
BioProject PRJNA1207524

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Supplementary file Size Download File type/resource
GSE286034_RAW.tar 94.4 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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