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Series GSE285980 Query DataSets for GSE285980
Status Public on Jan 07, 2025
Title AAV-mediated expression of proneural factors stimulates neurogenesis from adult Müller glia in vivo.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The lack of regeneration in the human central nervous system (CNS) has major health implications. To address this, we previously used transgenic mouse models to show that neurogenesis can be stimulated in the adult mammalian retina by driving regeneration programs that other species activate following injury. Expression of specific proneural factors in adult Müller glia causes them to re-enter the cell cycle and give rise to new neurons following retinal injury. To bring this strategy closer to clinical application, we now show that neurogenesis can also be stimulated when delivering these transcription factors to Müller glia using adeno-associated viral (AAV) vectors. AAV-mediated neurogenesis phenocopies the neurogenesis we observed from transgenic animals, with different proneural factor combinations giving rise to distinct neuronal subtypes in vivo. Vector-borne neurons are morphologically, transcriptomically and physiologically similar to bipolar and amacrine/ganglion-like neurons. These results represent a key step forward in developing a cellular reprogramming approach for regenerative medicine in the CNS.
 
Overall design Viral vectors expressing proneural genes can reliably stimulate glia of the adult mammalian retina to become various subtypes of neurons.
 
Contributor(s) Pavlou M, Probst M, Kaplan L, Filippova E, Prieve AR, Rieke F, Reh TA
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Submission date Jan 06, 2025
Last update date Jan 08, 2025
Contact name Thomas Reh
E-mail(s) tomreh@uw.edu
Organization name University of Washington
Department Department of Neurobiology and Biophysics
Street address 1959 NE Pacific Street, UW School of Medicine, Seattle, WA 98195
City Seattle
State/province Washington
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (10)
GSM8714395 untreated
GSM8714396 CBh>FLEX[Ascl1] (2w)
GSM8714397 CBh>FLEX[Ascl1-Atoh1] (2w)
Relations
BioProject PRJNA1207114

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE285980_RAW.tar 175.5 Mb (http)(custom) TAR (of H5)
SRA Run SelectorHelp
Raw data are available in SRA

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