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| Status |
Public on Aug 16, 2013 |
| Title |
DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis (RNA-seq) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) and small-interfering RNAs (siRNAs) negatively regulate their targets by 1) repressing translation, 2) endonucleolytic RNA cleavage, or 3) DNA methylation resulting in transcriptional silencing. P-body/decapping components are likely required for translational repression, but are not known to function in other posttranscriptional regulatory pathways or to affect smRNA levels. Here, we show that the P-body/decapping protein DCP5 is required for miRNA-mediated translational repression but not cleavage, and to regulate the transcription of specific miRNAs. We find that this protein also affects the abundance of tRNA-derived smRNAs. Significantly, DCP5 is required for the transcriptional silencing and DNA methylation of numerous transposable/repetitive elements and imprinted genes, indicating that it is a novel component of the RNA-directed DNA methylation pathway. Our results demonstrate that DCP5 and likely the P-body itself are required for multiple smRNA-mediated silencing pathways and provide the first evidence for the spatial separation of translational inhibition and cleavage by miRNAs.
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| Overall design |
total RNA expression comparison with between wildtype (Col-0) and dcp5 mutant plants in Arabidopsis
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| Contributor(s) |
Zheng Q, Endres MW, Dragomir I, Yang L, Weber R, Silverman IM, Poethig RS, Gregory BD |
| Citation(s) |
23150631 |
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| Submission date |
Apr 11, 2011 |
| Last update date |
Aug 16, 2013 |
| Contact name |
Qi Zheng |
| E-mail(s) |
qi.zheng@nemours.org
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| Phone |
3026515778
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| Organization name |
Nemours Children's Health
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| Street address |
1701 Rockland Road
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| City |
Wilmington |
| State/province |
DE |
| ZIP/Postal code |
19803 |
| Country |
USA |
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| Platforms (1) |
| GPL13396 |
AB SOLiD System (Arabidopsis thaliana) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE28524 |
DCP5, a highly conserved P-body-nucleating protein, regulates multiple small RNA-mediated silencing pathways in Arabidopsis |
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| Relations |
| BioProject |
PRJNA143055 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28509_RAW.tar |
1.3 Gb |
(http)(custom) |
TAR (of FASTA, TXT) |
| GSE28509_README.txt |
647 b |
(ftp)(http) |
TXT |
| Processed data provided as supplementary file |
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