|Public on Jun 01, 2011
|Hey3Met2 human ovarian cancer cells overexpressing wild type or catalytically-dead mutant RNASET2
|Expression profiling by array
|Hey3Met2 cells were stably transfected with plasmids encoding either wild-type RNASET2 or a catalytically dead form (whose cDNA has been previously mutagenized in the two CAS catalytic sites) or with the empty vector as a control. The control of ovarian tumorigenesis by RNASET2 occurs through modification of the cellular microenvironment and involvement of immunocompetent cells, thus providing evidence for specific modulations of cellular responses induced by RNASET2 that might underlay ovarian tumorigenesis. In order to get more inside on the effect of RNASET2 on the modulation of other genes, a whole genome expression has been run on Hey3Met2 cell transfected with wildtype or mutated RNASET2.
|Hey3Met2 human ovarian cancer cells were transfected with espression vectors encoding either wild type or catalitycally dead mutant RNASET2. Clones transfected with the empty vectors were used as negative controls. Three independent clones were used for the each type of transfected plasmid.
|Sacco MG, Fabbri M, Gribaldo L, Monti L, Lualdi M, Cinquetti R, Roumengous S, Acquati F, Taramelli R
|Apr 08, 2011
|Last update date
|Feb 22, 2018
|+39 0332 78 9538
|+39 0332 78 5446
|European Commission - Joint Research Centre
|Institute for Health and Consumer Protection
|Molecular Biology and Genomics unit
|Via E. Fermi, 2749
|Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)