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Series GSE28478 Query DataSets for GSE28478
Status Public on Jun 01, 2011
Title Hey3Met2 human ovarian cancer cells overexpressing wild type or catalytically-dead mutant RNASET2
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Hey3Met2 cells were stably transfected with plasmids encoding either wild-type RNASET2 or a catalytically dead form (whose cDNA has been previously mutagenized in the two CAS catalytic sites) or with the empty vector as a control. The control of ovarian tumorigenesis by RNASET2 occurs through modification of the cellular microenvironment and involvement of immunocompetent cells, thus providing evidence for specific modulations of cellular responses induced by RNASET2 that might underlay ovarian tumorigenesis. In order to get more inside on the effect of RNASET2 on the modulation of other genes, a whole genome expression has been run on Hey3Met2 cell transfected with wildtype or mutated RNASET2.
 
Overall design Hey3Met2 human ovarian cancer cells were transfected with espression vectors encoding either wild type or catalitycally dead mutant RNASET2. Clones transfected with the empty vectors were used as negative controls. Three independent clones were used for the each type of transfected plasmid.
 
Contributor(s) Sacco MG, Fabbri M, Gribaldo L, Monti L, Lualdi M, Cinquetti R, Roumengous S, Acquati F, Taramelli R
Citation(s) 21646684
Submission date Apr 08, 2011
Last update date Feb 22, 2018
Contact name marco fabbri
E-mail(s) marco.fabbri@jrc.ec.europa.eu
Phone +39 0332 78 9538
Fax +39 0332 78 5446
Organization name European Commission - Joint Research Centre
Department Institute for Health and Consumer Protection
Lab Molecular Biology and Genomics unit
Street address Via E. Fermi, 2749
City Ispra (VA)
ZIP/Postal code 21027
Country Italy
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (9)
GSM703814 Hey3Met2_control_rep1
GSM703815 Hey3Met2_control_rep2
GSM703816 Hey3Met2_control_rep3
Relations
BioProject PRJNA139305

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE28478_RAW.tar 88.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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