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Series GSE283889 Query DataSets for GSE283889
Status Public on Jan 06, 2025
Title Ly6ChiCD62LhiLy6GhiCx3CR1low Circulating Monocyte subsets are altered in a Murine Diabetic Gastroparesis Model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Circulating monocytes (Mo) are precursors to a subset of gastric resident-muscularis-macrophages (MMs). Changes in MMs are associated with delayed gastric emptying (DGE) in diabetic gastroparesis. Investigating the dynamics of Mo in an animal model of DGE using CyTOF and computational approaches, we show a high heterogeneity within the Mo-population. In DGE mice, via unbiased clustering we identified two reduced Mo clusters which exhibit migratory phenotype (Ly6ChiCD62LhiLy6GhiCD45RhiCCR2hi-intMERTEKhi-intCx3CR1low) resembling classical-Mo (CMo-like). All markers enriched in these clusters are known to regulate cell differentiation, proliferation, adhesion, and migration. Trajectory inference analysis predicted these Mo as precursors to subsequent Mo-lineages. In gastric muscle tissue, we demonstrated an increase in the gene expression level of chemokine receptor Ccr2, suggesting possibly increased trafficking of classical monocytes. These findings contribute to better understanding of the heterogenicity of the Mo-population and establish a link between two CMo-like clusters and the development of DGE phenotype.
 
Overall design In this study, we determined the state of Mo in the setting of diabetes with and without DGE in a STZ-induced-diabetes mouse model using cytometry by time-of-flight (CyTOF) and computational analysis.
 
Contributor(s) AlAsfoor S, Jessen E, Pullapantula SR, Voisin JR, Hsi LC, Pavelko KD, Farwana S, Ji S, Strausbauch MA, Cipriani G, Wei L, Linden DR, Hou R, Myers R, Bhattarai Y, Wykosky J, Burns AJ, Farrugia G, Dasari S, Grover M
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Submission date Dec 09, 2024
Last update date Jan 07, 2025
Contact name Madhusudan Grover
E-mail(s) grover.madhusudan@mayo.edu
Organization name Mayo Clinic
Street address 200 First St SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM8673888 Diabetic mouse 2416
GSM8673889 Delayed Gastric Emptying Diabetic mouse 2416
GSM8673890 Diabetic mouse 2860
Relations
BioProject PRJNA1195975

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE283889_RAW.tar 76.1 Mb (http)(custom) TAR (of H5)
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Raw data are available in SRA

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