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Status |
Public on Feb 28, 2013 |
Title |
Transcriptional profiles of MET transformed osteoblast clones (MET-HOB) and mesenchymal stem cells (MSC) where osteoblast differentiation was induced |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The osteogenic differentiation process consists in a well-coordinated multi-step sequence of events, in which each stage is characterized by the expression of distinct protein markers, as well as by individual morphological features. In order to precisely identify the step at which cells are permissive to MET-driven transformation, we used expression profiling. We compared the transcriptional profiles of MET-HOB and MSC cells where osteoblast differentiation was induced.
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Overall design |
Using microarrays, we evaluated the expression profiles of MET-HOB and MSC cells where osteoblast differentiation was induced, by culturing cells in the presence of ascorbic acid, α-glycerophosphate and dexamethasone for 7, 14 and 21 days
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Contributor(s) |
Dani N, Olivero M, Mareschi K, vanDuist M, Miretti S, Cuvertino S, Patané S, Calogero RA, Ferracini R, Scotlandi K, Fagioli F, DiRenzo M |
Citation missing |
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Submission date |
Mar 28, 2011 |
Last update date |
Mar 20, 2017 |
Contact name |
Raffaele A Calogero |
E-mail(s) |
raffaele.calogero@unito.it
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Phone |
++39 0116706454
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Organization name |
University of Torino
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Department |
Molecular Biotechnology Center
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Lab |
Bioinformatics and Genomics Unit
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Street address |
Via Nizza 52
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City |
Torino |
State/province |
To |
ZIP/Postal code |
10126 |
Country |
Italy |
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Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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Samples (16)
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GSM698427 |
mesenchymal stem cells [BM3_msc] |
GSM698428 |
osteoblast differentiation 7 days [BM3_7] |
GSM698429 |
osteoblast differentiation 14 days [BM3_14] |
GSM698430 |
osteoblast differentiation 7 days [BM5_7] |
GSM698431 |
osteoblast differentiation 14 days [BM5_14] |
GSM698432 |
osteoblast differentiation 21 days [BM5_21] |
GSM698433 |
mesenchymal stem cells [BM1_msc] |
GSM698434 |
osteoblast differentiation 21 days [BM1_21] |
GSM698435 |
osteoblast differentiation 7 days [BM2_7] |
GSM698436 |
osteoblast differentiation 14 days [BM2_14] |
GSM698437 |
mesenchymal stem cells [BM4_msc] |
GSM698438 |
osteoblast differentiation 7 days [BM4_7] |
GSM698439 |
mesenchymal stem cells [BM5_msc] |
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Relations |
BioProject |
PRJNA139645 |