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Series GSE281205 Query DataSets for GSE281205
Status Public on Jan 12, 2025
Title Clonally expanded targetable natural killer-like T cells seed the aged spinal cord to disrupt wound healing [scRNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The wound healing ability of the central nervous system declines with aging. Spinal cord injury (SCI) has been increasingly affecting aged individuals, where functional impairment and mortality are highest. However, the aging-dependent mechanisms underpinning tissue damage and repair after SCI remain elusive, limiting effective interventions. Here, we hypothesized that changes to the molecular and cellular signatures of the local spinal cord tissue microenvironment across the lifespan might affect spinal wound healing, axonal injury responses and functional recovery. To test this hypothesis, we investigated the molecular and cellular signatures associated with the wound healing response after an experimental SCI in the mouse across the lifespan by conducting single-cell RNAseq (scRNAseq) experiments from the spinal cord of young and aged mice preceding and following a SCI.
 
Overall design Young and aged mouse spinal cord segments were collected from conditions including naive, 14 days, and 28 days after spinal cord injury. After, cells were isolated for single cell RNAseq. Three biological replicates were included in each condition.
 
Contributor(s) Kong G, Zhou L, Di Giovanni S
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BioProject PRJNA1180700
Submission date Nov 06, 2024
Last update date Jan 13, 2025
Contact name Guiping Kong
E-mail(s) g.kong13@imperial.ac.uk
Organization name Imperial College London
Department Brain Sciences
Street address 55-96, Du Cane Road
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platforms (2)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM8614659 Young-naïve, replicate 1
GSM8614660 Young-naïve, replicate 2
GSM8614661 Young-naïve, replicate 3

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Supplementary file Size Download File type/resource
GSE281205_RAW.tar 2.1 Gb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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