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| Status |
Public on Sep 15, 2011 |
| Title |
COMPARISON OF DORSOCERVICAL TO ABDOMINAL SUBCUTANEOUS ADIPOSE TISSUE IN PATIENTS WITH AND WITHOUT ANTIRETROVIRAL THERAPY-ASSOCIATED LIPODYSTROPHY |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Background and objective: Combination antiretroviral therapy (cART) is associated with lipodystrophy i.e. loss of subcutaneous adipose tissue in abdomen, limbs and face and its accumulation intra-abdominally. No fat is lost dorsocervically and it can even accumulates in this region (“buffalo hump”). It is unknown how preserved dorsocervical fat differs from abdominal subcutaneous fat in HIV-1-infected cART-treated patients with (cART+LD+) and without (cART+LD-) lipodystrophy.
Results: Albeit dorsocervical adipose tissue in cART+LD+ seems spared from lipoatrophy, its mitochondrial DNA (mtDNA, copies/cell) content was significantly lower (by 62%) than that of the corresponding tissue in cART+LD-. Expression of CD68 mRNA, a marker of macrophages, and numerous inflammatory genes in microarray were significantly lower in dorsocervical vs. abdominal subcutaneous adipose tissue. Genes with the greatest difference in expression between the two depots were those involved in regulation of transcription and regionalization (homeobox genes), irrespective of lipodystrophy status. There was negligible mRNA expression of uncoupling protein 1, a gene characteristic of brown adipose tissue, in either depot.
Conclusions: As mtDNA is depleted even in the non-atrophic dorsocervical adipose tissue, it is unlikely that the cause of lipoatrophy is loss of mtDNA. Dorsocervical adipose tissue is less inflamed than lipoatrophic adipose tissue. It does not resemble brown adipose tissue. The greatest difference in gene expression between dorsocervical and abdominal s.c. adipose tissue is in expression of homeobox genes.
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| Overall design |
We used histology, microarray, polymerase chain reaction and magnetic resonance imaging to compare dorsocervical and abdominal subcutaneous adipose tissue in cART+LD+ (n=21) and cART+LD- (n=11).
The study consists of 17 patients on antiretroviral treatment who have developed lipodystrophy and 11 patients that are on similar treatment but have not developped lipodystrophy. All patients are HIV+ and have 2 adipose tissue samples taken from them (dorsocervical+abdominal).
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| Contributor(s) |
Sevastianova K, Sutinen J, Greco D, Sievers M, Salmenkivi K, Perttilä J, Olkkonen VM, Wågsäter D, Lidell ME, Enerbäck S, Eriksson P, Walker UA, Auvinen P, Ristola M, Yki-Järvinen H |
| Citation(s) |
21602514 |
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| Submission date |
Mar 21, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
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| Organization name |
Tampere University
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| Department |
Faculty of Medicine and Health Technology
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| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
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| Street address |
Arvo ylpön Katu 34
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| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (28)
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| GSM694182 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U1 |
| GSM694183 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U2 |
| GSM694184 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U4 |
| GSM694185 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U5 |
| GSM694186 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U6 |
| GSM694187 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U7 |
| GSM694188 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U8 |
| GSM694189 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U11 |
| GSM694190 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U12 |
| GSM694191 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U13 |
| GSM694192 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U14 |
| GSM694193 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U16 |
| GSM694194 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U17 |
| GSM694195 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U18 |
| GSM694196 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U19 |
| GSM694197 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U20 |
| GSM694198 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U22 |
| GSM694199 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U23 |
| GSM694200 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U24 |
| GSM694201 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U25 |
| GSM694202 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U26 |
| GSM694203 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U27 |
| GSM694204 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U28 |
| GSM694205 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U32 |
| GSM694206 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U33 |
| GSM694207 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U34 |
| GSM694208 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U35 |
| GSM694209 |
Dorsocervical vs. abdominal subcutaenous adipose tissue U36 |
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| Relations |
| BioProject |
PRJNA139717 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28073_RAW.tar |
291.5 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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