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Series GSE28073 Query DataSets for GSE28073
Status Public on Sep 15, 2011
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Background and objective: Combination antiretroviral therapy (cART) is associated with lipodystrophy i.e. loss of subcutaneous adipose tissue in abdomen, limbs and face and its accumulation intra-abdominally. No fat is lost dorsocervically and it can even accumulates in this region (“buffalo hump”). It is unknown how preserved dorsocervical fat differs from abdominal subcutaneous fat in HIV-1-infected cART-treated patients with (cART+LD+) and without (cART+LD-) lipodystrophy.
Results: Albeit dorsocervical adipose tissue in cART+LD+ seems spared from lipoatrophy, its mitochondrial DNA (mtDNA, copies/cell) content was significantly lower (by 62%) than that of the corresponding tissue in cART+LD-. Expression of CD68 mRNA, a marker of macrophages, and numerous inflammatory genes in microarray were significantly lower in dorsocervical vs. abdominal subcutaneous adipose tissue. Genes with the greatest difference in expression between the two depots were those involved in regulation of transcription and regionalization (homeobox genes), irrespective of lipodystrophy status. There was negligible mRNA expression of uncoupling protein 1, a gene characteristic of brown adipose tissue, in either depot.
Conclusions: As mtDNA is depleted even in the non-atrophic dorsocervical adipose tissue, it is unlikely that the cause of lipoatrophy is loss of mtDNA. Dorsocervical adipose tissue is less inflamed than lipoatrophic adipose tissue. It does not resemble brown adipose tissue. The greatest difference in gene expression between dorsocervical and abdominal s.c. adipose tissue is in expression of homeobox genes.
Overall design We used histology, microarray, polymerase chain reaction and magnetic resonance imaging to compare dorsocervical and abdominal subcutaneous adipose tissue in cART+LD+ (n=21) and cART+LD- (n=11).
The study consists of 17 patients on antiretroviral treatment who have developed lipodystrophy and 11 patients that are on similar treatment but have not developped lipodystrophy. All patients are HIV+ and have 2 adipose tissue samples taken from them (dorsocervical+abdominal).
Contributor(s) Sevastianova K, Sutinen J, Greco D, Sievers M, Salmenkivi K, Perttilä J, Olkkonen VM, Wågsäter D, Lidell ME, Enerbäck S, Eriksson P, Walker UA, Auvinen P, Ristola M, Yki-Järvinen H
Citation(s) 21602514
Submission date Mar 21, 2011
Last update date Feb 22, 2018
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (28)
GSM694182 Dorsocervical vs. abdominal subcutaenous adipose tissue U1
GSM694183 Dorsocervical vs. abdominal subcutaenous adipose tissue U2
GSM694184 Dorsocervical vs. abdominal subcutaenous adipose tissue U4
BioProject PRJNA139717

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Supplementary file Size Download File type/resource
GSE28073_RAW.tar 291.5 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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