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Series GSE28040 Query DataSets for GSE28040
Status Public on Sep 21, 2011
Title Accurate Identification of A-to-I RNA editing in human by transcriptome sequencing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA editing enhances the diversity of gene products at the post-transcriptional level. Approaches for genome-wide identification of RNA editing face two main challenges: separating true editing sites from false discoveries and accurate estimation of editing levels. We developed an approach to analyze transcriptome sequencing data (RNA-Seq) for global identification of RNA editing in cells for which whole-genome sequencing data are available. We applied the method to analyze RNA-Seq data of a human glioblastoma cell line, U87MG. Around 10,000 DNA-RNA differences were identified, the majority being putative A-to-I editing sites. These predicted A-to-I events were associated with a low false discovery rate (~5%). Moreover, the estimated editing levels from RNA-Seq correlated well with those based on traditional clonal sequencing. Our results further facilitated unbiased characterization of the sequence and evolutionary features flanking predicted A-to-I editing sites and discovery of a conserved RNA structural motif that may be functionally relevant to editing. Genes with predicted A-to-I editing were significantly enriched with those known to be involved in cancer, supporting the potential importance of cancer-specific RNA editing. A similar profile of DNA-RNA differences as in U87MG was predicted for another RNA-Seq data set obtained from primary breast cancer samples. Remarkably, significant overlap exists between the putative editing sites of the two transcriptomes despite their difference in cell type, cancer type and genomic backgrounds. Our approach enabled de novo identification of the RNA editome, which sets the stage for further mechanistic studies of this important step of post-transcriptional regulation.
Overall design Examine mRNA expression in U87MG cells following ADAR1 or control siRNA knockdown
Contributor(s) Bahn J, Lee J, Li G, Greer C, Peng G, Xiao X
Citation(s) 21960545
Submission date Mar 18, 2011
Last update date May 15, 2019
Contact name Gang Li
Organization name UCLA
Street address 611 Charles E. Young Drive Rm. 660
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (2)
GSM693746 u87_adar_ctrl
GSM693747 u87_adar_kd
SRA SRP009659
BioProject PRJNA137951

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Supplementary file Size Download File type/resource
GSE28040_RAW.tar 4.3 Gb (http)(custom) TAR (of TAR)
GSE28040_README.txt 576 b (ftp)(http) TXT
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Processed data provided as supplementary file
Raw data are available in SRA

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