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Series GSE27960 Query DataSets for GSE27960
Status Public on May 22, 2011
Title Sensing prokaryotic mRNA signifies microbial viability and promotes immunity
Organism Mus musculus
Experiment type Expression profiling by array
Summary MyD88-independent signal transduction associated with Toll-like receptors (TLRs) 3 and TLR4 is mediated through the adapter protein TRIF (TIR-domain-containing adapter-inducing interferon-beta). It has been proposed that TLR signalling is important for the transcription of crucial inflammasome components like NLRP3, a process that has been termed "priming". In order to test whether TRIF signalling was required for the priming of inflammasome components, we performed a genome wide transcriptional analysis on wild-type and Trif-knockout bone marrow derived macrophages (BMMs) before and 1, 3 and 6 hours after phagocytosis of E. coli. These results indicated that TRIF was involved in the activation and not transcriptional priming of the NLRP3 inflammasome.
Overall design Bone marrow derived macrophages from WT and Trif knockout mice, stimulated with E.coli for up to 6hrs.
Contributor(s) Sander LE, Davis MJ, Boekschoten MV, Amsen D, Dasher CC, Ryffel B, Swanson JA, Müller M, Blander JM
Citation(s) 21602824, 27348412
Submission date Mar 15, 2011
Last update date Mar 30, 2020
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (24)
GSM691419 macrophage_wildtype_control_rep1
GSM691420 macrophage_wildtype_control_rep2
GSM691421 macrophage_wildtype_control_rep3
BioProject PRJNA137747

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Supplementary file Size Download File type/resource
GSE27960_RAW.tar 111.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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