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| Status |
Public on Nov 15, 2024 |
| Title |
Single nucleus RNA sequencing of mosiac Pax2 and Pax8 mutant proximal tubules |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Pax2 and Pax8 are homologous transcription factors required for kidney development and medullary urine concentration. However, their function in proximal tubule homeostasis and response to acute kidney injury is unknown. Mice with proximal tubules consisting of a mosaic of wild-type and Pax2/8 mutant proximal tubules cells were generated. Gene expression of mutant and wild-type proximal tubule cells was compared under homeostatic conditions using single-nucleus RNA sequencing.
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| Overall design |
Female mice heterozygous for the X-linked phosphoenolpyruvate carboxykinase Cre driver and conditional Pax2, Pax8, and tdTomato/green fluorescent protein reporter alleles were generated. The proximal tubules of these mice were composed of wild-type and Pax2/8 mutant cells due to random X-inactivation. 12-week-old uninjured mice were euthanized, and their kidneys were flash-frozen for single-nucleus RNA sequencing.
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| Contributor(s) |
Beamish JA |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K08 DK125776 |
Molecular genetic mechanisms of renal cell regeneration |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Jeffrey Alan Beamish |
| R01 DK054740 |
Pax2 Interacting Proteins in Development and Disease |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Gregory R Dressler |
| R01 DK073722 |
Epigenetic Regulation of Kidney Development |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Gregory R Dressler |
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| Submission date |
Sep 27, 2024 |
| Last update date |
Nov 16, 2024 |
| Contact name |
Jeffrey A Beamish |
| E-mail(s) |
jebeamis@med.umich.edu
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| Organization name |
University of Michigan
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| Department |
Department of Internal Medicine, Division of Nephrology
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| Street address |
1500 E. Medical Center Drive, SPC 5364
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1166220 |
