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| Status |
Public on Oct 30, 2024 |
| Title |
Transcriptomic landscape of mammalian ventral pallidum at single-cell resolution |
| Organisms |
Macaca mulatta; Papio anubis; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ventral pallidum (VP) is critical for motivated behaviors. While contemporary work has begun to elucidate the functional diversity of VP neurons, the molecular heterogeneity underlying this functional diversity remains incompletely understood. We used snRNA-seq and in situ hybridization to define the transcriptional taxonomy of VP cell types in mice, macaques, and baboons. We found transcriptional conservation between all three species, within the broader neurochemical cell types. Unique dopaminoceptive and cholinergic subclusters were identified and conserved across both primate species but had no homolog in mice. This harmonized consensus VP cellular atlas will pave the way for understanding the structure and function of the VP and identified key neuropeptides, neurotransmitters, and neuro receptors that could be targeted within specific VP cell types for functional investigations.
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| Overall design |
VP tissues were dissected from C57Bl6J mice, olive baboons, and rhesus macaque. Nuclear suspension was isolated by FACS based on 7-AAD signal and processed by 10X Genomics.
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| Contributor(s) |
Yang L, Fang L, Lynch MR, Xu CS, Hahm H, Zhang Y, Heitmeier MR, Costa V, Samineni VK, Creed MC |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 18, 2024 |
| Last update date |
Oct 30, 2024 |
| Contact name |
Lite Yang |
| E-mail(s) |
yanglite1211@gmail.com
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| Organization name |
Washington University in St. Louis
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| Street address |
4922 Parkview Place
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
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| Platforms (3) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL27943 |
Illumina NovaSeq 6000 (Macaca mulatta) |
| GPL30489 |
Illumina NovaSeq 6000 (Papio anubis) |
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| Samples (17)
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| GSM8522774 |
Mouse, female sample2, sublibrary1 |
| GSM8522775 |
Mouse, male sample2, sublibrary1 |
| GSM8522776 |
Baboon, male subject1, right hemisphere, sublibrary1 |
| GSM8522777 |
Baboon, male subject1, left hemisphere, sublibrary1 |
| GSM8522778 |
Baboon, male subject2, right hemisphere, sublibrary1 |
| GSM8522779 |
Baboon, male subject2, left hemisphere, sublibrary1 |
| GSM8522780 |
Baboon, male subject2, left hemisphere, sublibrary2 |
| GSM8522781 |
Baboon, male subject2, left hemisphere, sublibrary3 |
| GSM8522782 |
Macaque, female subject1, left hemisphere, sublibrary1 |
| GSM8522783 |
Macaque, male subject1, left hemisphere, sublibrary1 |
| GSM8522784 |
Macaque, female subject2, right hemisphere, sublibrary1 |
| GSM8522785 |
Macaque, female subject2, left hemisphere, sublibrary1 |
| GSM8522786 |
Macaque, female subject2, left hemisphere, sublibrary2 |
| GSM8522787 |
Macaque, female subject2, left hemisphere, sublibrary3 |
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| Relations |
| BioProject |
PRJNA1162478 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE277465_baboon_gene_matrix.rds.gz |
255.8 Mb |
(ftp)(http) |
RDS |
| GSE277465_baboon_metadata.csv.gz |
739.3 Kb |
(ftp)(http) |
CSV |
| GSE277465_macaque_gene_matrix.rds.gz |
248.1 Mb |
(ftp)(http) |
RDS |
| GSE277465_macaque_metadata.csv.gz |
647.0 Kb |
(ftp)(http) |
CSV |
| GSE277465_mouse_gene_matrix.rds.gz |
169.8 Mb |
(ftp)(http) |
RDS |
| GSE277465_mouse_metadata.csv.gz |
750.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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