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Series GSE275211 Query DataSets for GSE275211
Status Public on Sep 06, 2024
Title Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We examined the transcriotional changes in antigen specific CD4 T cells to antigens derived from thymus or to the antigens derived from islets in the autoimmune diabetes mouse model.
 
Overall design Single cells were prepared from spleen and lymph nodes of 8-12 weeks old NOD mice or HEL specfic TCR transgenic mice. Tetramer enriched cells were sorted from the NOD mice and naïve CD4 T cells were sorted from HEL TCR trangenic mice. Before surface staining, samples were incubated with 100 μl of TotalSeq™ anti-mouse Hashtag reagent (1:100 [vol/vol], BioLegend) to tag the samples: enriched cells from the islet epitope set were tagged with the TotalSeq-C0301 anti-mouse Hashtag 1 Antibody (Barcode sequence: ACCCACCAGTAAGAC); enriched cells from the thymic epitope set were tagged with the TotalSeq-C0302 anti-mouse Hashtag 2 Antibody (Barcode sequence: GGTCGAGAGCATTCA); 10E11 naïve CD4+ T cells were tagged with the TotalSeq-C0303 anti-mouse Hashtag 3 Antibody (Barcode sequence: CTTGCCGCATGTCAT).
 
Contributor(s) Hu H, Li T, Zhang B, Wan X
Citation(s) 39333495
Submission date Aug 19, 2024
Last update date Dec 12, 2024
Contact name Tiandao Li
Organization name Washington University
Street address 4444 Forest Park Ave
City St Louis
State/province MO
ZIP/Postal code 63108
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM8472799 Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 1
GSM8472800 Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 2
Relations
BioProject PRJNA1149809

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Supplementary file Size Download File type/resource
GSE275211_RAW.tar 63.9 Mb (http)(custom) TAR (of TAR)
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Raw data are available in SRA

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