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Status |
Public on Sep 06, 2024 |
Title |
Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We examined the transcriotional changes in antigen specific CD4 T cells to antigens derived from thymus or to the antigens derived from islets in the autoimmune diabetes mouse model.
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Overall design |
Single cells were prepared from spleen and lymph nodes of 8-12 weeks old NOD mice or HEL specfic TCR transgenic mice. Tetramer enriched cells were sorted from the NOD mice and naïve CD4 T cells were sorted from HEL TCR trangenic mice. Before surface staining, samples were incubated with 100 μl of TotalSeq™ anti-mouse Hashtag reagent (1:100 [vol/vol], BioLegend) to tag the samples: enriched cells from the islet epitope set were tagged with the TotalSeq-C0301 anti-mouse Hashtag 1 Antibody (Barcode sequence: ACCCACCAGTAAGAC); enriched cells from the thymic epitope set were tagged with the TotalSeq-C0302 anti-mouse Hashtag 2 Antibody (Barcode sequence: GGTCGAGAGCATTCA); 10E11 naïve CD4+ T cells were tagged with the TotalSeq-C0303 anti-mouse Hashtag 3 Antibody (Barcode sequence: CTTGCCGCATGTCAT).
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Contributor(s) |
Hu H, Li T, Zhang B, Wan X |
Citation(s) |
39333495 |
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Submission date |
Aug 19, 2024 |
Last update date |
Dec 12, 2024 |
Contact name |
Tiandao Li |
Organization name |
Washington University
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Street address |
4444 Forest Park Ave
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City |
St Louis |
State/province |
MO |
ZIP/Postal code |
63108 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (2) |
GSM8472799 |
Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 1 |
GSM8472800 |
Pooled cells with thymus specific, islet specific, and HEL specific T cells_replicate 2 |
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Relations |
BioProject |
PRJNA1149809 |