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Series GSE275179 Query DataSets for GSE275179
Status Public on Aug 22, 2024
Title Adult skull bone marrow is an expanding and resilient hematopoietic reservoir
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The bone marrow microenvironment is a critical regulator of hematopoietic stem cell self-renewal and fate. While it is appreciated that aging, chronic inflammation and other insults compromise bone marrow function and thereby negatively affect hematopoiesis, it is not known whether different bone compartments exhibit distinct microenvironmental properties and functional resilience. Here, we have employed imaging, pharmacological approaches, and mouse genetics to uncover specialized and highly surprising properties of bone marrow in adult and aging skull. Specifically, we show that the skull bone marrow undergoes lifelong expansion involving vascular growth, which results in an increasing contribution to total hematopoietic output. Furthermore, skull is largely protected against major hallmarks of aging, including upregulation of pro-inflammatory cytokines, adipogenesis and loss of vascular integrity. Striking dynamic and rapid changes to the skull vasculature and bone marrow are induced by physiological alterations, namely pregnancy, but also pathological challenges, such as stroke and experimental chronic myeloid leukemia. These responses are highly distinct from femur, the most extensively studied bone marrow compartment. We propose that skull harbors a protected and dynamically expanding bone marrow microenvironment, which is relevant for experimental studies but, potentially, also clinical treatments in humans.
 
Overall design FACS-sorted hematopoietic stem and progenitor cells (HSPCs) and MACS hematopoietic lineage-depleted stromal cells from skull or femoral bone marrow of young or old mice analyzed using scRNA-seq
 
Contributor(s) Koh BI, Jeong H, Kruse K, Adams RH
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Submission date Aug 19, 2024
Last update date Aug 23, 2024
Contact name Kai Kruse
E-mail(s) bioinformatics-service@mpi-muenster.mpg.de
Organization name Max Planck Institute for Molecular Biomedicine
Department Bioinformatics Core Facility
Street address Roentgenstr. 20
City Münster
State/province North Rhine-Westphalia
ZIP/Postal code 48149
Country Germany
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (19)
GSM8472468 Old Femur All Replicate 1
GSM8472469 Old Skull All Replicate 1
GSM8472470 Old Femur Stromal
Relations
BioProject PRJNA1149727

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE275179_BD_CLS1.txt.gz 412 b (ftp)(http) TXT
GSE275179_BD_CLS1_v2.txt.gz 1.3 Kb (ftp)(http) TXT
GSE275179_BD_CLS2.txt.gz 417 b (ftp)(http) TXT
GSE275179_BD_CLS2_v2.txt.gz 1.3 Kb (ftp)(http) TXT
GSE275179_BD_CLS3.txt.gz 415 b (ftp)(http) TXT
GSE275179_BD_CLS3_v2.txt.gz 1.3 Kb (ftp)(http) TXT
GSE275179_RAW.tar 4.5 Gb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA

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