|
| Status |
Public on Oct 06, 2011 |
| Title |
Gene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The exchange of the oocyte’s genome with the genome of a somatic cell, followed by the derivation of pluripotent stem cells, could enable the generation of specific cell types affected in degenerative human diseases. Such cells, carrying the patient’s genome, might be useful for cell replacement. Here we report that the development of human oocytes activated after genome exchange invariably arrests at the late cleavage stages in association with transcriptional abnormalities. In contrast, if the oocyte genome is not removed and the somatic cell genome is merely added, they efficiently develop to the blastocyst stage. Human stem cell lines derived from these blastocysts differentiate into cell types of all three germ layers, and a pluripotent gene expression program is established on the genome derived from the somatic cell. This result demonstrates the feasibility of reprogramming human cells using oocytes and identifies the removal of the oocyte genome as the primary cause of developmental failure after genome exchange. Future work should focus on the critical elements that are associated with the human oocyte genome.
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| Overall design |
Stem cells were derived by reprogramming of skin cells using oocytes ('nuclear transfer') or defined factors (iPS cells), or from IVF blastocysts
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| Contributor(s) |
Egli D |
| Citation(s) |
21979046 |
| Submission date |
Feb 24, 2011 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Dieter Egli |
| E-mail(s) |
degli@nyscf.org
|
| Phone |
617 4968659
|
| Organization name |
The New York Stem Cell Foundation Research Institute
|
| Department |
NYSCF
|
| Lab |
Egli
|
| Street address |
3960 Broadway
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
|
| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE28024 |
Human oocytes reprogram somatic cells to a pluripotent state |
|
| Relations |
| BioProject |
PRJNA141865 |
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