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Series GSE274951 Query DataSets for GSE274951
Status Public on Jan 07, 2025
Title Transcriptional acclimation to warming temperatures in the Australian alpine herb Wahlenbergia ceracea
Organism Wahlenbergia ceracea
Experiment type Expression profiling by high throughput sequencing
Summary Understanding the molecular basis of plant heat tolerance helps to predict the consequences of climate warming on plant performance, particularly in vulnerable environments. Our current understanding comes primarily from studies in Arabidopsis thaliana (Arabidopsis) and select crops exposed to short and intense periods of heat stress. In this study we sought to characterise the transcriptomic responses of Wahlenbergia ceracea (W. ceracea), an Australian alpine herb, to growth under sustained moderate warming. We compared responses of pre-defined tolerant and sensitive lines, based on measures of photosynthetic thermostability, to growth under cool (24/15 ºC day/night) or warm (30/20 ºC day/night) temperatures to identify the pathways involved in heat tolerance and acclimation. Under warmer growth temperatures, W. ceracea up-regulated genes involved in RNA metabolism, while down-regulating those involved in photosynthesis and pigment metabolism. In tolerant lines, genes related to photosystem II and light-dependent photosynthetic reactions were more strongly down-regulated. This suggests that the regulation of electron transport and its components may be involved in thermal acclimation. Our results also highlight the importance of hormonal gene networks, particularly those responsive to ethylene, during longer-term moderate warming. In conclusion, our results point to post-transcriptional processes and the stabilisation of the electron transport chain as candidate mechanisms for thermal acclimation in heat-tolerant W. ceracea. The study also revealed many temperature-responsive genes with no detectable ortholog in Arabidopsis, whose characterization may enhance our understanding of physiological acclimation and have relevance for the biotechnological improvement of threatened species and crops.
 
Overall design To investigate transcriptional responses potentially involved in physiological acclimation to warming, we grew plants ofWahlenbergia ceraceaunder either cool (current) or warmer (future) temperatures
We then performed differential gene expression analysis using RNA-seq data from leaf tissues harvested after two weeks of acclimation under experimental conditions
 
Contributor(s) Notarnicola RF, Arnold PA, Feng Z, Hamilton J, Jones AW, Loke S, Schwessinger B, Ganguly DR, Nicotra AB
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Submission date Aug 15, 2024
Last update date Jan 08, 2025
Contact name Rocco Fabrizio Notarnicola
E-mail(s) roccofabrizio.notarnicola2@gmail.com
Organization name Adam Mickiewicz University
Department Evolutionary Biology
Street address ul, Uniwersytetu Poznańskiego 6
City Poznan
State/province Wielkopolskie
ZIP/Postal code 61-614
Country Poland
 
Platforms (1)
GPL34820 Illumina NovaSeq 6000 (Wahlenbergia ceracea)
Samples (72)
GSM8462687 Leaf, Warm, 437, rep 1
GSM8462688 Leaf, Warm, 439, rep 1
GSM8462689 Leaf, Cool, 457, rep 1
Relations
BioProject PRJNA1148632

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE274951_RAW.tar 209.4 Mb (http)(custom) TAR (of H5, TSV)
GSE274951_count_matrix.csv.gz 11.5 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA

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