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Series GSE273759 Query DataSets for GSE273759
Status Public on Aug 31, 2024
Title Dynamic phosphorylation of FOXA1 by Aurora B guides post-mitotic gene reactivation
Organism Homo sapiens
Experiment type Other
Summary FOXA1 serves as a crucial pioneer transcription factor in developmental processes, and plays a pivotal role as a mitotic bookmarking factor to perpetuate gene expression profiles and maintain cellular identity. During mitosis, the majority of FOXA1 dissociates from specific DNA binding sites and redistributes to non-specific binding sites, however, the regulatory mechanisms governing FOXA1's molecular dynamics in mitosis remain elusive. Here we show that mitotic kinase Aurora B regulates FOXA1's different DNA binding modes and the formation of the biomolecular condensate. Mechanistically, Aurora B kinase phosphorylates FOXA1 at Serine 221 (S221) to disrupt the specific, but not the non-specific, DNA binding. The phosphorylation of S221 also negatively regulates the FOXA1 condensates that require specific DNA binding. Importantly, perturbation of the dynamic phosphorylation impairs accurate gene reactivations and cell proliferation, suggesting that reversible mitotic protein phosphorylation emerges as a fundamental mechanism for the spatiotemporal control of mitotic bookmarking.
 
Overall design CUT&Tag sequencing for FOXA1 wild-type and FOXA1 S221A in interphase and mitotic stably expressing GFP-FOXA1 wild-type and S221A HeLa cell
 
Contributor(s) Zhang T, Liu S, Liu X, Yao X
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Submission date Aug 01, 2024
Last update date Aug 31, 2024
Contact name 张 婷
E-mail(s) jocelynz@mail.ustc.edu.cn
Phone 18895364168
Organization name 中国科学技术大学
Street address 安徽省合肥市蜀山区黄山路443号中国科学技术大学西校区生命科学学院547室
City hefei
State/province Select an option…
ZIP/Postal code 230027
Country China
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (7)
GSM8436518 Interphase HeLa, GFP, control, CUT&Tag (C1)
GSM8436519 Interphase HeLa, GFP-FOXA1, WT-group1, CUT&Tag (K3)
GSM8436520 Interphase HeLa, GFP-FOXA1, WT-group2, CUT&Tag (K4)
Relations
BioProject PRJNA1142976

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Supplementary file Size Download File type/resource
GSE273759_RAW.tar 250.3 Mb (http)(custom) TAR (of BW, TXT)
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Raw data are available in SRA

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