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| Status |
Public on Jul 28, 2024 |
| Title |
Transcriptome data of B cells and T helper cells from patients with multiple sclerosis receiving therapeutic apheresis for the treatment of an acute relapse |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We analyzed the transcriptome profiles of CD19+ B cells and CD4+ T cells from the peripheral blood of patients with multiple sclerosis (MS) immediately before and after relapse therapy with apheresis. High-density Clariom D Arrays for human were used to obtain gene-level and exon-level expression profiles. This GEO entry provides the processed data from the gene-level and exon-level workflows.
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| Overall design |
Peripheral blood was taken from 4 MS patients before the first (T0) and after the last (T1) session of apheresis (i.e., plasma exchange or immunoadsorption) for the treatment of an acute exacerbation.
CD19+ B cells were isolated from 3 blood sample pairs, while CD4+ T helper cells were isolated from 4 blood sample pairs, resulting in a total of 14 samples. Total RNA was extracted, labeled and hybridized to Clariom D microarrays to quantify the transcript levels.
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| Contributor(s) |
Hecker M, Koczan D, Zettl UK |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 24, 2024 |
| Last update date |
Jul 29, 2024 |
| Contact name |
Michael Hecker |
| E-mail(s) |
michael.hecker@rocketmail.com
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| Organization name |
University of Rostock
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| Department |
Department of Neurology
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| Lab |
Division of Neuroimmunology
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| Street address |
Gehlsheimer Str. 20
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| City |
Rostock |
| ZIP/Postal code |
18147 |
| Country |
Germany |
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| Platforms (1) |
| GPL23126 |
[Clariom_D_Human] Affymetrix Human Clariom D Assay [transcript (gene) version] |
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| Samples (14)
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| GSM8415859 |
patient C2P9, before apheresis, B cells |
| GSM8415860 |
patient C2P9, after apheresis, B cells |
| GSM8415861 |
patient C2P9, before apheresis, T cells |
| GSM8415862 |
patient C2P9, after apheresis, T cells |
| GSM8415863 |
patient C4P3, before apheresis, B cells |
| GSM8415864 |
patient C4P3, after apheresis, B cells |
| GSM8415865 |
patient C4P3, before apheresis, T cells |
| GSM8415866 |
patient C4P3, after apheresis, T cells |
| GSM8415867 |
patient C4P4, before apheresis, B cells |
| GSM8415868 |
patient C4P4, after apheresis, B cells |
| GSM8415869 |
patient C4P4, before apheresis, T cells |
| GSM8415870 |
patient C4P4, after apheresis, T cells |
| GSM8415871 |
patient C4P5, before apheresis, T cells |
| GSM8415872 |
patient C4P5, after apheresis, T cells |
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| Relations |
| BioProject |
PRJNA1139737 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE272973_GA_affy_Transcriptomes_MS_APH_exon-level_Matrix.txt.gz |
65.5 Mb |
(ftp)(http) |
TXT |
| GSE272973_RAW.tar |
555.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
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