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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 26, 2024 |
| Title |
Targeting fatty acid metabolism to abrogate differentiation block in pre-leukemia induced by AML1-ETO |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To gain the underlying insight into the functional impact of AML1-ETO expressed in HSPCs, RNA-seq was performed on purified LT-HSCs and GMPs from the bone marrow (BM) of both AML1-ETO expressed (AML1/ETO) and Wild-Type (control) C57 mice. Our work suggested that AML1-ETO resulted in impaired hematopoietic reconstitution and increased self-renewal ability.The oxidative phosphorylation and glycolysis decreased significantly in AML1/ETO LT-HSCs accompanied by increased HSC quiescence and reduced cell cycling. Furthermore, it was observed that HSCs expressing AML1-ETO exhibited an increased requirement for fatty acids when they differentiated.
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| Overall design |
Expression profiling by high throughput sequencing of LT-HSC cells and GMP cells for AML1/ETO mice and control mice
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| Contributor(s) |
Wang J, Qiu S, Liu X |
| Citation(s) |
39264725 |
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| Submission date |
Jun 21, 2024 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Yu Liu |
| E-mail(s) |
liuyu@ihcams.ac.cn
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| Organization name |
Chinese Academy of Medical Sciences & Peking Union Medical College
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| Street address |
288 Nanjing Road
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| City |
Tianjin |
| State/province |
-- None selected -- |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (14)
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| GSM8343094 |
LT-HSC cells, Control mouse,rep1 |
| GSM8343095 |
LT-HSC cells, Control mouse,rep2 |
| GSM8343096 |
LT-HSC cells, Control mouse,rep3 |
| GSM8343097 |
LT-HSC cells, Control mouse,rep4 |
| GSM8343098 |
GMP cells, AML1/ETO mouse, rep1 |
| GSM8343099 |
GMP cells, AML1/ETO mouse, rep2 |
| GSM8343100 |
GMP cells, AML1/ETO mouse, rep3 |
| GSM8343101 |
GMP cells, Control mouse,rep1 |
| GSM8343102 |
GMP cells, Control mouse,rep2 |
| GSM8343103 |
GMP cells, Control mouse,rep3 |
| GSM8343104 |
GMP cells, Control mouse,rep4 |
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| Relations |
| BioProject |
PRJNA1126647 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE270447_BulkRNAseq_gene_count.txt.gz |
609.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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