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Status |
Public on Jun 26, 2024 |
Title |
Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth [scRNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Here, we sought to understand the function of stromal interleukin-33 (IL-33) in PanIN and PDA. We collected pancreatic tissues from various mouse models of PanIN and PDA for single cell RNA sequencing to assess IL-33 expression across the microenviornment. We also implanted syngeneic orthotopic pancreatic tumors into mice harboring a conditional stromal IL-33 knockout and sequenced the resulting tumors.
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Overall design |
Pancreata from various mouse models of pancreatic tumorigenesis (spontaneous or orthotopic injection) collected and subjected to single cell RNA sequencing.
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Contributor(s) |
Donahue KL, Zhang Y, Lasse-Opsahl EL, Pasca di Magliano M |
Citation(s) |
38958646 |
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Submission date |
Jun 14, 2024 |
Last update date |
Oct 15, 2024 |
Contact name |
Marina Pasca di Magliano |
E-mail(s) |
marinapa@umich.edu
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Organization name |
University of Michigan Medical School
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Street address |
1500 E Medical Center Dr
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City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48103 |
Country |
USA |
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Platforms (2) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (11)
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Relations |
BioProject |
PRJNA1124072 |