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| Status |
Public on Jul 29, 2024 |
| Title |
A replisome-associated parental histone H3-H4 tetramer chaperone required for faithful epigenetic inheritance |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Other
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| Summary |
Faithful transfer of parental histones to newly replicated daughter DNA strands is critical for inheritance of epigenetic states. Although replication proteins that facilitate parental histone transfer have been identified, how intact histone H3-H4 tetramers travel relatively large distances from the front to the back of the replication fork remains unknown. Here, we use AlphaFold-Multimer structural predictions combined with biochemical and genetic approaches to identify the Mrc1/CLASPIN subunit of the replisome as a histone chaperone. Mrc1 contains a conserved histone binding domain that forms a brace around the H3-H4 tetramer mimicking nucleosomal DNA and H2A-H2B histones, is required for heterochromatin inheritance, and promotes parental histone recycling during replication. We further identify binding sites for the FACT histone chaperone in Swi1/TIMELESS and DNA polymerase α that are required for heterochromatin inheritance. We propose that Mrc1, in concert with FACT acting as a mobile co-chaperone, coordinates the distribution of parental histones to newly replicated DNA.
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| Overall design |
eSPAN (enrichment and sequencing of protein-associated nascent DNA) experiments to detect the strand bias of the distribution of parental histone surrogate H3K4me3 at the replication origins in S. pombe
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| Contributor(s) |
Juntao Y, Yimeng F, Xu H, Zhiguo Z, Songtao J, Danesh M |
| Citation(s) |
39094570 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM072805 |
Epigenetic inheritance of heterochromatin |
HARVARD UNIVERSITY (MEDICAL SCHOOL) |
Danesh Moazed |
| R35 GM118015 |
Mechanism of Epigenetic Inheritance |
Trustees of Columbia University in the City of New York |
Zhiguo Zhang |
| R35 GM126910 |
Mechanism of Heterochromatin Assembly and epigenetic inheritance |
The Trustees of Columbia University in the City of New York |
Songtao Jia |
| R01 GM067945 |
New Sample Multiplexing Technologies to Identify Chemical Probes and Illuminate Ubiquitin Biology |
HARVARD UNIVERSITY (MEDICAL SCHOOL) |
Steven P Gygi |
| R01 GM132129 |
Advancing Multiplexed Isobaric Tag-based Strategies for Proteome Profiling |
HARVARD UNIVERSITY (MEDICAL SCHOOL) |
Joao A Paulo |
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| Submission date |
Jun 07, 2024 |
| Last update date |
Aug 13, 2024 |
| Contact name |
Danesh Moazed |
| E-mail(s) |
danesh@hms.harvard.edu
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| Organization name |
Harvard Medical School/HHMI
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| Street address |
240 Longwood Avenue, LHRRB 517
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL20584 |
Illumina NextSeq 500 (Schizosaccharomyces pombe) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1121313 |
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