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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 26, 2024 |
| Title |
PI3Kg in B cells promotes antibody responses and generation of antibody-secreting cells [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The differentiation of naïve and memory B cells into antibody-secreting cells (ASCs) is a key feature of adaptive immunity. The requirement for phosphoinositide 3-kinase delta (PI3Kδ) to support B cell biology has been investigated intensively; however, specific functions of the related phosphoinositide 3-kinase-gamma (PI3Kγ) complex in B-lineage cells have not. Here we report that PI3Kγ promotes robust antibody responses induced by T cell-dependent antigens. The inborn error of immunity caused by human deficiency in PI3Kγ results in broad humoral defects, prompting our investigation of roles for this kinase in antibody responses using mouse models with bulk and single-cell transcriptome as well as B cell receptor repertoire sequencing.
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| Overall design |
For single-cell RNA and B cell receptor sequencing, lymph node cells were analyzed after enrichment for B cell subsets from either wild-type or Pik3cg knockout mice (n = 3 each) immunized three times with NP-Ova emulsified in alum (Day 34). Cells from the different mice were barcoded with mouse TotalSeqTM-C (BioLegend) before mixing and sequencing using two identical lanes.
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| Contributor(s) |
Lanahan SM, Barmada A, Ramaswamy A, Gabernet G, Lucas CL |
| Citation(s) |
38961274 |
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| Submission date |
Jun 06, 2024 |
| Last update date |
Jul 04, 2024 |
| Contact name |
Anis Barmada |
| Organization name |
Yale University
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| Department |
Department of Immunobiology
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| Lab |
Carrie Lucas Lab
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| Street address |
300 Cedar Street
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1120924 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE269300_RAW.tar |
111.3 Mb |
(http)(custom) |
TAR (of MTX, TSV, TXT) |
| GSE269300_TotalSeq-CITE-barcodes-to-genotype.csv.gz |
143.0 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
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