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Series GSE26866 Query DataSets for GSE26866
Status Public on Jan 30, 2012
Title Combined Use of Laser Capture Microdissection and Microarray Analysis Identifies Locally Expressed Disease-Related Genes in Focal Regions of Psoriasis Vulgaris Skin Lesions
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this study, we sought to establish the usefulness of LCM on cDNA microarray analysis. We reported that LCM samples improved the sensitivity of detection of differentially expressed genes over conventional bulk tissue analysis. We also provided the new information of chemokine and its receptor interaction within psoriatic lesional skin.
 
Overall design For the comparison of gene expression patterns between single and double amplification techniques, total RNA was extracted from blocks of paired lesional and non-lesional psoriatic skin biopsies from four patients.
For LCM experiment, total RNA was extracted from sliced tissue sections of paired lesional and non-lesional psoriatic skin biopsies from three patients.
 
Contributor(s) Mitsui H, Suárez-Fariñas M, Krueger JG
Citation(s) 22402443
Submission date Jan 25, 2011
Last update date Dec 06, 2018
Contact name Mayte Suarez-Farinas
E-mail(s) mayte.suarezfarinas@mssm.edu
Organization name Mount SinaiSchool of Medicine
Street address 1425 Madison Ave, L2-70C, Box 1077,
City New York
State/province NY
ZIP/Postal code 10075
Country USA
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (37)
GSM661382 1_NL_whole_Single
GSM661387 1_LS_whole_Single
GSM661389 2_NL_whole_Single
Relations
BioProject PRJNA135995

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26866_RAW.tar 74.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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