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| Status |
Public on Jun 28, 2024 |
| Title |
Organoid Culture Promotes Dedifferentiation of Mouse Myoblasts into Stem Cells Capable of Complete Muscle Regeneration |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Experimental cell therapies for skeletal muscle conditions have shown little success, primarily because they used committed myogenic progenitors rather than true muscle stem cells, known as satellite cells. Here we present a method to generate in vitro–derived satellite cells (idSCs) from skeletal muscle tissue. When transplanted in small numbers into mouse muscle, mouse idSCs fuse into myofibers, repopulate the satellite cell niche, self-renew, support multiple rounds of muscle regeneration, and improve force production on par with freshly isolated satellite cells. in damaged skeletal muscle. We profiled the epigenomic and transcriptional differences between idSCs and satellite cells, and used these differences to identify core signaling pathways and genes that confer idSC functionality. Finally, fromhuman muscle biopsies we successfully generated satellite cell–like cells in vitro. After further development, idSCs may provide a scalable source of cells for the treatment of genetic muscle disorders, trauma-induced muscle damage and age-related muscle weakness.
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| Overall design |
ATAC-seq was performed on mouse satellite cells, myoblasts, and in vitro-derived satellite cells at 10, 20, and 30 days of generation.
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| Contributor(s) |
Rubin LL, Price FD, Gehrke AR, Holton KM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 20, 2024 |
| Last update date |
Jun 28, 2024 |
| Contact name |
Kristina M Holton |
| E-mail(s) |
kmholton@g.harvard.edu
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| Organization name |
Harvard University
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| Department |
HSCRB
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| Lab |
Lee Rubin Lab
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| Street address |
7 Divinity Ave
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA1113694 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE267952_D10_IDR.narrowPeak.gz |
2.0 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE267952_D10_merge.bw |
743.9 Mb |
(ftp)(http) |
BW |
| GSE267952_D20_IDR.narrowPeak.gz |
2.3 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE267952_D20_merge.bw |
942.1 Mb |
(ftp)(http) |
BW |
| GSE267952_D30_IDR.narrowPeak.gz |
1.7 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE267952_D30_merge.bw |
917.4 Mb |
(ftp)(http) |
BW |
| GSE267952_MYO_IDR.narrowPeak.gz |
2.4 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE267952_MYO_merge.bw |
935.0 Mb |
(ftp)(http) |
BW |
| GSE267952_SC_IDR.narrowPeak.gz |
1.4 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE267952_SC_merge.bw |
1.0 Gb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
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