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Series GSE26740 Query DataSets for GSE26740
Status Public on Jun 02, 2011
Title Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer [expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Estrogen receptor α (ERα) is key player in the progression of breast cancer. ERα binds to DNA and mediates long-range chromatin interactions throughout the genome, but the underlying mechanism in this process is unclear. Here, we show that AP-2 motifs are highly enriched in the ERα binding sites (ERBS) identified from the recent ChIA-PET of ERα. More importantly, we demonstrate that AP-2γ (also known as TFAP2C), a member of the AP-2 family which has been implicated in breast cancer oncogenesis, is recruited to chromatin in a ligand-independent manner and co-localized with ERα binding events. Furthermore, pertubation of AP-2γ expression disrupts ERα DNA binding, long-range chromatin interactions, and gene transcription. Using ChIP-seq, we show that AP-2γ and ERα binding occurs in close proximity on a genome-wide scale. The majority of these shared genomic regions are also occupied by the pioneer factor, FoxA1. AP-2γ is required for efficient FoxA1 binding and vice versa. Finally, we show that most ERBS associated with long-range chromatin interactions are co-localized with both AP-2γ and FoxA1. Together, our results suggest AP-2γ is an essential factor in ERα-mediated transcription, primarily working together with FoxA1 to facilitate ERα binding and long-range chromatin interactions.
 
Overall design Gene expression profiling of negative control (NC) and AP-2γ siRNA transfected MCF-7, with and without E2 (estradiol) stimulation using microarray.
 
Contributor(s) Tan SK, Lin Z, Chang CW, Cheung E
Citation(s) 21572391
Submission date Jan 20, 2011
Last update date Mar 20, 2017
Contact name SI KEE TAN
E-mail(s) tansk99@gis.a-star.edu.sg
Organization name Genomic Institute of Singapore
Department Cancer biology and Pharmacology
Street address 60 Biopolis Street #02-01 Genome
City Singapore
ZIP/Postal code Singapore 138672
Country Singapore
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (6)
GSM658197 NC-transfected MCF-7, 12h ETOH
GSM658198 NC-transfected MCF-7, 12h E2
GSM658199 siAP-2γ (Dharmacon)-transfected MCF-7, 12h ETOH
This SubSeries is part of SuperSeries:
GSE26741 Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer
Relations
BioProject PRJNA142129

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE26740_RAW.tar 3.9 Mb (http)(custom) TAR
GSE26740_non-normalized.txt.gz 6.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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